Choi Sung-In, Lee Hyun-Ah, Han Ji-Sook
Department of Food Science and Nutrition, Pusan National University, Jangjeon 2-dong, Geumjeong-gu, Busan 46241, Korea.
Department of Food Science and Nutrition, Pusan National University, Jangjeon 2-dong, Geumjeong-gu, Busan 46241, Korea.; Research Institute of Ecology for the Elderly, Pusan National University, Busan 46241, Korea.
Nutr Res Pract. 2016 Oct;10(5):507-515. doi: 10.4162/nrp.2016.10.5.507. Epub 2016 Jun 16.
BACKGROUND/OBJECTIVES: This study was designed to investigate whether extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.
MATERIALS/METHODS: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.
Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.
These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.
背景/目的：本研究旨在探讨葡萄皮提取物（GPE）能否在2型糖尿病动物模型中改善胰岛素敏感性并抑制肝糖生成。
材料/方法：将C57BL/Ksj-db/db小鼠分为3组，分别给予常规饮食（对照组）、GPE和罗格列酮（0.005 g/100 g饮食），喂养6周。
补充GPE的小鼠血糖和糖化血红蛋白水平显著低于糖尿病对照小鼠。葡萄糖和胰岛素耐量试验也显示GPE对提高胰岛素敏感性有积极作用。补充GPE的小鼠胰岛素抵抗稳态指数显著低于糖尿病对照小鼠。与糖尿病对照小鼠相比，补充GPE的小鼠骨骼肌中磷酸化的AMP激活蛋白激酶、160 kDa的pAkt底物和质膜葡萄糖转运蛋白4的表达增加。GPE还降低了肝脏中葡萄糖-6-磷酸酶和磷酸烯醇式丙酮酸羧激酶的表达。
这些发现表明，GPE可能改善胰岛素敏感性并抑制肝脏中的糖异生。
