Yang L Q, Zhang Y, Sun H F
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China.
Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China
Genet Mol Res. 2016 Aug 26;15(3):gmr8364. doi: 10.4238/gmr.15038364.
Our study aimed to investigate the role of 2 ERCC5 promoter SNPs (rs2094258 and rs751402) in the development of gastric cancer in the Chinese population. The present hospital-based case-control study consisted of 155 patients with gastric cancer and 246 healthy controls recruited between March 2012 and December 2014. Genotyping for the rs2094258 and rs751402 polymorphic sites was carried out using polymerase chain reaction-restriction fragment length polymorphism. Statistical analyses were conducted using the SPASS version 16.0 software (SPSS, Inc., Chicago, IL, USA). As determined by the chi-square test, there was a significant difference in the genotype distributions of rs751402 between patients and controls (X = 6.74, P = 0.03). By unconditional logistic regression analysis, we observed that the TT genotype in rs751402 was significantly associated with increased risk to gastric cancer as compared with the CC genotype, and the adjusted OR (95%CI) was 2.17 (1.15-4.09). Moreover, subjects carrying the T allele in rs751402 had elevated risk of developing gastric cancer when compared with those carrying the C allele, with an adjusted OR value (95%CI) of 1.47 (1.09-1.99). In conclusion, we suggest that the ERCC5 rs751402 gene polymorphism may influence the susceptibility to gastric cancer in the Chinese population.
我们的研究旨在调查2个ERCC5启动子单核苷酸多态性（rs2094258和rs751402）在中国人群胃癌发生中的作用。本基于医院的病例对照研究纳入了2012年3月至2014年12月期间招募的155例胃癌患者和246例健康对照。使用聚合酶链反应-限制性片段长度多态性方法对rs2094258和rs751402多态性位点进行基因分型。使用SPASS 16.0软件（美国伊利诺伊州芝加哥市SPSS公司）进行统计分析。经卡方检验确定，患者和对照之间rs751402的基因型分布存在显著差异（X = 6.74，P = 0.03）。通过无条件逻辑回归分析，我们观察到与CC基因型相比，rs751402中的TT基因型与胃癌风险增加显著相关，调整后的比值比（95%置信区间）为2.17（1.15 - 4.09）。此外，与携带C等位基因的受试者相比，携带rs751402中T等位基因的受试者患胃癌的风险升高，调整后的比值比（95%置信区间）为1.47（1.09 - 1.99）。总之，我们认为ERCC5 rs751402基因多态性可能影响中国人群对胃癌的易感性。
