一种用于评估乙烯环氧化酶活性的气相色谱法。
A gas chromatographic method for the evaluation of the vinyl epoxidase activity.
作者信息
Malvoisin E, Rollmann B, Roberfroid M, Mercier M
出版信息
Arch Toxicol Suppl. 1978(1):299-302. doi: 10.1007/978-3-642-66896-8_60.
Two alternative specific and very sensitive methods for determination of vinyl epoxide synthetase activity in liver microsomes are described. Trichloroethylene, which is used as a substrate, is converted into trichloroethylene oxide by a hepatic epoxide synthetase. Chloral hydrate, the final rearrangement product of trichloroethylene oxide, is evaluated by electron capture gas chromatography, either after derivatization with pentafluorophenyl-hydrazine or after its conversion into chloroform under alcaline conditions. The kinetic parameters of the epoxidative reaction have been determined on rat hepatic microsomal suspensions.
本文描述了两种用于测定肝微粒体中环氧乙烯合成酶活性的特异性强且非常灵敏的替代方法。用作底物的三氯乙烯被肝脏环氧合成酶转化为三氯环氧乙烷。三氯环氧乙烷的最终重排产物水合氯醛，可通过电子捕获气相色谱法进行评估，评估方法为在与五氟苯基肼衍生化后，或在碱性条件下将其转化为氯仿后进行评估。已在大鼠肝微粒体悬浮液上测定了环氧化反应的动力学参数。
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