Laboratory for Simulation, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 103, Freiburg im Breisgau 79110, Germany.
School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Lab Chip. 2016 Oct 5;16(20):4002-4011. doi: 10.1039/c6lc00973e.
Typical Lab-on-a-Disc (LoaD) platforms cannot make a continuous measurement while the disc is spinning; this drawback means that the disc usually must be stopped and aligned with a sensor. This can result in measurement errors in time-dependent assays along with inaccuracies due to liquid displacement and bubble formation in the absence of a stabilising centrifugal field. This paper presents a novel concept for a wirelessly electrified-Lab-on-a-Disc (eLoaD) platform that allows continuous measurement of experimental parameters while the disc is spinning. This platform incorporates all the components needed for measurement within the rotating frame of reference, and bidirectional transmission of data outside this reference frame, thus allowing for online measurement independent of the rotation of the disc. The eLoaD platform is conceived in a modular manner whereby an interchangeable and non-disposable 'Application Disc' can be fitted to the eLoaD platform and so the system can be adapted for a range of optical, electrochemical and other measurement types. As an application example, optical readout, using the Application Disc fitted with a silicon photomultiplier, is demonstrated using a tagged chemiluminescent antibody, which is commonly used, for instance, in ELISA assays. The precision of the eLoaD platform is >94%, while its accuracy, when compared to a commercial benchtop luminometer, is higher than 96%. The modular design of this platform will permit extension of this technology to many other LoaD applications.
典型的盘上实验室（LoaD）平台在盘旋转时无法进行连续测量；这一缺点意味着盘通常必须停止并与传感器对齐。这可能导致时间依赖测定中的测量误差，并且在没有稳定离心场的情况下，由于液体位移和气泡形成而导致不准确。本文提出了一种新颖的无线通电盘上实验室（eLoaD）平台概念，该平台允许在盘旋转时连续测量实验参数。该平台将测量所需的所有组件集成在旋转参考系内，并在该参考系外双向传输数据，从而允许在线测量与盘的旋转无关。eLoaD 平台采用模块化设计，可在 eLoaD 平台上安装可互换且不可丢弃的“应用盘”，从而使系统能够适应各种光学、电化学和其他测量类型。作为应用示例，使用装有硅光电倍增管的应用盘进行光学读出，使用标记的化学发光抗体进行演示，该抗体常用于 ELISA 测定等。eLoaD 平台的精度>94%，与商用台式发光计相比，其准确性高于 96%。该平台的模块化设计将允许将这项技术扩展到许多其他 LoaD 应用。
