Laboratory for Simulation, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 103, Freiburg im Breisgau 79110, Germany; Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany.
Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany.
Biosens Bioelectron. 2018 Oct 15;117:464-473. doi: 10.1016/j.bios.2018.05.056. Epub 2018 Jun 5.
In this paper we present a comprehensive description of the design, fabrication and operation of an electrified Lab-on-a-Disc (eLoaD) system. The smart platform is developed to extend conventional Lab-on-a-Disc applications with an electronic interface, providing additional flow control and sensing capabilities to centrifugal microfluidics platforms. Wireless power is transferred from a Qi-compliant transmitter to the eLoaD platform during rotation. An Arduino-based microcontroller, a Bluetooth communication module, and an on-board SD-card are integrated into the platform. This generalises the applicability of the eLoaD and its modules for performing a wide range of laboratory unit operations, procedures, or diagnostic assays, all controlled wirelessly during spinning. The lightweight platform is fully reusable and modular in design and construction. An interchangeable and non-disposable application disc is fitted with the necessary sensors and/or actuators for a specific assay or experiment to be performed. A particular advantage is the ability to continuously monitor and interact with LoaD experiments, overcoming the limitations of stroboscopy. We demonstrate the applicability of the platform for three sensing experiments involving optical, electrochemical, and temperature detection, and one actuation experiment involving controlled heating/cooling. The complete electronic designs and example programming codes are extensively documented in the supplementary material for easy adaptation.
本文全面介绍了电动盘上实验室（eLoaD）系统的设计、制造和运行。该智能平台是为了在传统的盘上实验室应用中增加电子接口而开发的，为离心微流控平台提供了额外的流量控制和传感功能。在旋转过程中，无线电源从符合 Qi 标准的发射器传输到 eLoaD 平台。一个基于 Arduino 的微控制器、一个蓝牙通信模块和一个板载 SD 卡被集成到平台中。这使得 eLoaD 及其模块的适用性得到了推广，可以在旋转过程中无线控制执行广泛的实验室单元操作、程序或诊断分析。该平台重量轻，设计和构造完全可重复使用和模块化。一个可互换的、不可重复使用的应用盘配备了执行特定分析或实验所需的必要传感器和/或执行器。一个特别的优点是能够连续监测和与 LoaD 实验进行交互，克服了频闪观测的限制。我们展示了该平台在三个涉及光学、电化学和温度检测的传感实验以及一个涉及受控加热/冷却的执行实验中的适用性。完整的电子设计和示例编程代码在补充材料中都有详细记录，便于进行适应性调整。
