Sedgwick Adam C, Sun Xiaolong, Kim Gyoungmi, Yoon Juyoung, Bull Steven D, James Tony D
Department of Chemistry, University of Bath, BA2 7AY, UK.
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Chem Commun (Camb). 2016 Oct 11;52(83):12350-12352. doi: 10.1039/c6cc06829d.
A simple probe for the detection of peroxynitrite was developed incorporating a benzyl boronic ester "protecting" unit. The "protecting" unit of the probe is removed by peroxynitrite to "turn-on" ESIPT fluorescence (4.5 fold enhancement). Furthermore, the probe was cell permeable and was used in cell imaging experiments showing an off-on response towards peroxynitrite, in HeLa and RAW 264.7 cells.
开发了一种用于检测过氧亚硝酸盐的简单探针，该探针包含一个苄基硼酸酯“保护”单元。过氧亚硝酸盐会去除探针的“保护”单元，从而“开启”激发态分子内质子转移（ESIPT）荧光（增强4.5倍）。此外，该探针具有细胞渗透性，并用于细胞成像实验，在HeLa细胞和RAW 264.7细胞中显示出对过氧亚硝酸盐的开启响应。
