间充质干细胞与内皮祖细胞联合移植用于组织工程：一项系统评价与荟萃分析
Combined transplantation of mesenchymal stem cells and endothelial progenitor cells for tissue engineering: a systematic review and meta-analysis.
作者信息
Sun Kunming, Zhou Zheng, Ju Xinxin, Zhou Yang, Lan Jiaojiao, Chen Dongdong, Chen Hongzhi, Liu Manli, Pang Lijuan
机构信息
Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases (Ministry of Education), Shihezi University School of Medicine, North 2nd Road, Shihezi, 832002, Xinjiang, China.
Department of Pathology, First Affiliated Hospital to Shihezi University School of Medicine, Shihezi, China.
出版信息
Stem Cell Res Ther. 2016 Oct 10;7(1):151. doi: 10.1186/s13287-016-0390-4.
BACKGROUND
Combined cell implantation has been widely applied in tissue engineering in recent years. In this meta-analysis, we aimed to establish whether the combined transplantation of mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) promotes angiogenesis and tissue repair, compared with transplantation of a single cell type, following tissue injury or during tissue regeneration.
METHODS
The electronic databases PubMed, EMBASE, MEDLINE, Chinese Biomedical Literature, and China National Knowledge Infrastructure were searched in this systematic review and meta-analysis. Eighteen controlled preclinical studies involving MSC and EPC transplantation in animal models of disease, or in coculture in vitro, were included in this review. The vessel density and other functional indexes, which were classified according to the organ source, were used to evaluate the efficiency of cotransplantation. Publication bias was assessed.
RESULTS
There was no obvious difference in angiogenesis following combined cell transplantation (EPCs and MSCs) and transplantation of EPCs alone; however, an improvement in the function of damaged organs was observed following cotransplantation. In addition, combined cell transplantation significantly promoted tissue recovery in cardiovascular disease, cerebrovascular disease, and during bone regeneration. Compared with combined transplantation (EPCs and MSCs) and transplantation of MSCs alone, cotransplantation significantly promoted angiogenesis and bone regeneration, as well as vessel revascularization and tissue repair in cerebrovascular disease; however, no obvious effects on cardiovascular disease were observed.
CONCLUSIONS
As an exploratory field in the discipline of tissue engineering, MSC and EPC cotransplantation offers advantages, although it is essential to assess the feasibility of this approach before clinical trials can be performed.
背景
近年来，联合细胞植入已广泛应用于组织工程领域。在本荟萃分析中，我们旨在确定在组织损伤或组织再生过程中，与单一细胞类型移植相比，间充质干细胞（MSC）和内皮祖细胞（EPC）联合移植是否能促进血管生成和组织修复。
方法
在本系统评价和荟萃分析中，检索了电子数据库PubMed、EMBASE、MEDLINE、中国生物医学文献数据库和中国知网。本评价纳入了18项涉及在疾病动物模型中或体外共培养中进行MSC和EPC移植的对照临床前研究。根据器官来源分类的血管密度和其他功能指标用于评估联合移植的效果。评估发表偏倚。
结果
联合细胞移植（EPC和MSC）和单独移植EPC后的血管生成没有明显差异；然而，联合移植后观察到受损器官功能有所改善。此外，联合细胞移植显著促进了心血管疾病、脑血管疾病和骨再生过程中的组织恢复。与联合移植（EPC和MSC）和单独移植MSC相比，联合移植显著促进了血管生成和骨再生，以及脑血管疾病中的血管再通和组织修复；然而，对心血管疾病没有观察到明显影响。
结论
作为组织工程学科中的一个探索性领域，MSC和EPC联合移植具有优势，尽管在进行临床试验之前评估这种方法的可行性至关重要。
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