Song Yonggui, Su Dan, Shen Yuan, Liu Hongyu, Wang Li
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang, Jiangxi, 330022, China.
Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang, Jiangxi, 330006, China.
Anal Bioanal Chem. 2017 Jan;409(1):161-168. doi: 10.1007/s00216-016-9982-1. Epub 2016 Oct 11.
A novel open circuit potential biosensor (OCPS) composed of a working electrode and a Ag/AgCl reference electrode was designed for in vivo continuous glucose monitoring in this work. The macroporous carbon derived from kenaf stem (KSC) was used to construct a KSC microelectrode (denoted as KSCME) which was subsequently used to load glucose oxidase (GOD) as the working electrode. The resulting GOD/KSCMEs could catalyze the oxidation of glucose directly to result in changes of the open circuit potential (V ) of the OCPS. The V of OCPS was dependent on the glucose concentration, showing a linear range of 0.03-10.0 mM (R = 0.999) with a detection limit of 10 μM. In addition, the OCPS exhibited good selectivity for glucose over other common endogenous interferences. The feasibility of the proposed OCPS for glucose detection in mice skin tumors and normal tissue homogenate samples (in vitro experiment) and rat subcutaneous glucose monitoring (in vivo experiment) was also demonstrated with satisfactory results. The biosensor represents a novel example of a superficial cancer diagnostic device, and the proposed OCPS also provides new ideas for the development of a simple and highly selective device for continuous glucose sensing.
在本研究中，设计了一种由工作电极和Ag/AgCl参比电极组成的新型开路电位生物传感器（OCPS），用于体内连续葡萄糖监测。采用源自红麻茎的大孔碳构建KSC微电极（记为KSCME），随后将其用作负载葡萄糖氧化酶（GOD）的工作电极。所得的GOD/KSCMEs可直接催化葡萄糖氧化，导致OCPS的开路电位（V）发生变化。OCPS的V取决于葡萄糖浓度，线性范围为0.03 - 10.0 mM（R = 0.999），检测限为10 μM。此外，OCPS对葡萄糖具有良好的选择性，优于其他常见的内源性干扰物。所提出的OCPS用于小鼠皮肤肿瘤和正常组织匀浆样品中葡萄糖检测（体外实验）以及大鼠皮下葡萄糖监测（体内实验）的可行性也得到了验证，结果令人满意。该生物传感器代表了一种新型的浅表癌症诊断装置实例，所提出的OCPS也为开发一种用于连续葡萄糖传感的简单且高选择性装置提供了新思路。
