Batista-Duharte Alexander, Jorge Murillo Gisela, Pérez Ulpiano Marqués, Tur Enieyis Naranjo, Portuondo Deivys Fuentes, Martínez Bruno Téllez, Téllez-Martínez Damiana, Betancourt Juan E, Pérez Oliver
Toxicology and Biomedicine Center (TOXIMED), Medical Science University of Santiago de Cuba, Santiago de Cuba, Cuba
São Paulo Estate University (UNESP), Pharmaceutical Sciences Faculty, Araraquara, Brazil.
Int J Toxicol. 2016 Nov;35(6):627-633. doi: 10.1177/1091581816672187. Epub 2016 Oct 12.
Local reactions are the most frequent adverse event associated with vaccines. Adjuvants are major constituents of many vaccines and they are frequently involved in these reactions, associated with their irritating effect and the stimulation of local inflammation. The hen's egg test on chorioallantoic membrane (HET-CAM) is an alternative toxicological method widely used to determine ocular irritation potential, but very few studies have demonstrated the utility of this method for assessing the irritant properties of vaccine adjuvants. In this work, known/experimental adjuvants were evaluated by both HET-CAM and an in vivo local toxicity study in mice to compare irritation scores to determine whether there was a correlation (Pearson test). Based on these data (r = 0.9034; P < 0.0001), the HET-CAM assay can be used as an alternate method for the prediction of the local toxicity potential of adjuvant candidates to be used in vaccines.
局部反应是与疫苗相关的最常见不良事件。佐剂是许多疫苗的主要成分，它们经常参与这些反应，这与其刺激作用和局部炎症的激发有关。鸡胚绒毛尿囊膜试验（HET-CAM）是一种广泛用于确定眼刺激潜力的替代毒理学方法，但很少有研究证明该方法在评估疫苗佐剂刺激特性方面的效用。在这项工作中，通过HET-CAM和小鼠体内局部毒性研究对已知/实验性佐剂进行评估，以比较刺激评分，从而确定是否存在相关性（Pearson检验）。基于这些数据（r = 0.9034；P < 0.0001），HET-CAM试验可作为预测疫苗中使用的佐剂候选物局部毒性潜力的替代方法。
