Park Jeong-Ann, Kim Song-Bae
Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
Environmental Functional Materials and Water Treatment Laboratory, Seoul National University, Seoul 08826, Republic of Korea; Department of Rural Systems Engineering/Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Chemosphere. 2017 Jan;167:469-477. doi: 10.1016/j.chemosphere.2016.10.030. Epub 2016 Oct 14.
Antimicrobial electrospun poly(vinyl alcohol) (PVA) nanofibers were synthesized by impregnating benzyl triethylammonium chloride (BTEAC) as an antimicrobial agent into PVA nanofibers. The BTEAC-PVA nanofibers were heat-methanol treated during the preparation for various tests. The BTEAC-PVA nanofibers became more hydrophilic than the PVA nanofibers due to incorporation of BTEAC. Through heat-methanol treatment, thermal property, crystallinity, and water stability of BTEAC-PVA nanofibers were improved considerably. The immersion test shows that heat-methanol treatment has an advantage over heat treatment to maintain BTEAC content in BTEAC-PVA nanofibers. The acute toxicity test demonstrates that the 24-h EC and 48-h EC values (EC = median effective concentration) of BTEAC to Daphnia magna were 113 and 90 mg/L, respectively. The leaching test indicates that the BTEAC concentration leached from BTEAC-PVA nanofibers was far below the concentration affecting the immobilization of D. magna. For antimicrobial filtration tests, the BTEAC-PVA nanofibers were deposited onto glass fiber filter. The antimicrobial filtration test was conducted against bacteria (Escherichia coli, Staphylococcus aureus) and bacteriophages (MS2, PhiX174), demonstrating that the BTEAC-PVA nanofibers could enhance the removal of E. coli and S. aureus considerably but not the removal of MS2 and PhiX174 under dynamic flow conditions.
通过将作为抗菌剂的苄基三乙基氯化铵（BTEAC）浸渍到聚乙烯醇（PVA）纳米纤维中，合成了抗菌电纺PVA纳米纤维。在制备用于各种测试的过程中，对BTEAC-PVA纳米纤维进行了热甲醇处理。由于BTEAC的掺入，BTEAC-PVA纳米纤维比PVA纳米纤维更具亲水性。通过热甲醇处理，BTEAC-PVA纳米纤维的热性能、结晶度和水稳定性得到了显著改善。浸泡试验表明，热甲醇处理在保持BTEAC-PVA纳米纤维中BTEAC含量方面比热处理更具优势。急性毒性试验表明，BTEAC对大型溞的24小时EC值和48小时EC值（EC = 半数有效浓度）分别为113和90 mg/L。浸出试验表明，从BTEAC-PVA纳米纤维中浸出的BTEAC浓度远低于影响大型溞固定的浓度。对于抗菌过滤试验，将BTEAC-PVA纳米纤维沉积到玻璃纤维过滤器上。针对细菌（大肠杆菌、金黄色葡萄球菌）和噬菌体（MS2、PhiX174）进行了抗菌过滤试验，结果表明，在动态流动条件下，BTEAC-PVA纳米纤维可以显著提高对大肠杆菌和金黄色葡萄球菌的去除率，但对MS2和PhiX174的去除率没有提高。
