Nanshan District Key Lab for Biopolymers and Safety Evaluation, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, PR China.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110855. doi: 10.1016/j.msec.2020.110855. Epub 2020 Mar 14.
Bacterial adhesion and colonization on material surfaces have attracted great attention due to their potential threat to human health. Combining bactericidal and antifouling functions has been confirmed as an optimal strategy to prevent microbial infection. In this work, biodegradable electrospun polyvinyl alcohol (PVA) nanofibers were chosen due to its high specific area and abundant reactive hydroxyl groups. A quaternary ammonium salt (IQAS) and zwitterionic sulfopropylbetaine (ISB), both containing isocyanate (NCO) groups, were chemically bonded to the PVA nanofiber surface via a coupling reaction between the OH groups of the PVA nanofibers and the NCO groups of IQAS or ISB. The results indicated that the antimicrobial rates of PVA nanofibers modified by IQAS (0.5%) reached 99.9% against both gram-positive Staphylococcus aureus (S. aureus, ATCC 6538) and gram-negative Escherichia coli (E. coli, ATCC 25922). Additionally, the live/dead staining and cytotoxicity test indicated that the dual functional IQAS/ISB/PVA nanofibers exhibited excellent bactericidal and antifouling activities with low cytotoxicity. This work may provide practical guidelines to fabricate bactericidal and antifouling materials for healthcare applications, including but not limited to wound dressings, textile, food packaging and air filtration.
由于细菌在材料表面的黏附和定植会对人类健康造成潜在威胁，因此人们对其给予了高度关注。将杀菌和抗污功能相结合已被证实是预防微生物感染的最佳策略。在这项工作中，由于具有高比表面积和丰富的反应性羟基，选择了可生物降解的静电纺丝聚乙烯醇（PVA）纳米纤维。季铵盐（IQAS）和两性离子磺丙基甜菜碱（ISB）都含有异氰酸酯（NCO）基团，通过 PVA 纳米纤维上的 OH 基团与 IQAS 或 ISB 的 NCO 基团之间的偶联反应，化学结合到 PVA 纳米纤维表面上。结果表明，经 IQAS（0.5%）修饰的 PVA 纳米纤维对革兰氏阳性金黄色葡萄球菌（S. aureus，ATCC 6538）和革兰氏阴性大肠杆菌（E. coli，ATCC 25922）的抗菌率均达到 99.9%。此外，活/死染色和细胞毒性试验表明，具有双重功能的 IQAS/ISB/PVA 纳米纤维具有出色的杀菌和抗污活性，同时细胞毒性低。这项工作可能为医疗保健应用（包括但不限于伤口敷料、纺织品、食品包装和空气过滤）中杀菌和抗污材料的制备提供实用指南。
