Pournaqi Faeze, Ghiaee Armaghan, Vakilian Saeid, Ardeshirylajimi Abdolreza
Department of Biology, Faculty of Science, Islamic Azad University of Tehran East, Tehran, Iran.
Stem Cell Technology Research Center, Tehran, Iran.
Biologicals. 2017 Jan;45:78-84. doi: 10.1016/j.biologicals.2016.09.010. Epub 2016 Oct 14.
Tissue engineering is a promising emerged method trying to reconstruct lost tissues that using synthetic and biomaterials and their combination with cells. The purpose of this study is increase osteoinductivity of polyethersulfone (PES) by using polyaniline (PANi). In this study, after fabrication of PES and composited PES-PANi scaffolds by electrospinning, scaffolds were characterized morphologically and mechanically. Then osteoinductivity of scaffolds was investigated by osteogenic differentiation of human mesenchymal stem cells (MSCs) cultured on the PES and PES-PANI in comparison to the tissue culture polystyrene as a control. The Osteogenic potential of MSCs was evaluated by Alizarin Red staining, ALP activity, calcium content assay and bone related gene expression assay. Scaffolds were smooth, bead free and in the scale of nanometers and PES mechanical stability was decreased significantly after composite with PANi. Highest growth, ALP activity and deposited calcium of cells were observed in the PES-PANi groups compared to the PES and TCPS. Significantly higher expression of collagen type one and osteonectin was also detected in the PES-PANi group. It can be concluded that PES-PANi construct has potential to be a good candidate as bone grafting substitute and using in tissue engineering applications.
组织工程是一种很有前景的新兴方法，试图利用合成材料、生物材料及其与细胞的组合来重建受损组织。本研究的目的是通过使用聚苯胺（PANi）提高聚醚砜（PES）的骨诱导性。在本研究中，通过静电纺丝制备PES和复合PES-PANi支架后，对支架进行形态学和力学表征。然后，与作为对照的组织培养聚苯乙烯相比，通过在PES和PES-PANI上培养的人间充质干细胞（MSCs）的成骨分化来研究支架的骨诱导性。通过茜素红染色、碱性磷酸酶（ALP）活性、钙含量测定和骨相关基因表达测定来评估MSCs的成骨潜力。支架表面光滑、无珠粒且尺寸在纳米级别，与PANi复合后，PES的机械稳定性显著降低。与PES和组织培养聚苯乙烯（TCPS）相比，在PES-PANi组中观察到细胞的生长、ALP活性和钙沉积最高。在PES-PANi组中还检测到I型胶原蛋白和骨连接蛋白的表达显著更高。可以得出结论，PES-PANi构建体有潜力成为骨移植替代物的良好候选者，并用于组织工程应用。
