Jo Peter, Nietert Manuel, Gusky Linda, Kitz Julia, Conradi Lena C, Müller-Dornieden Annegret, Schüler Philipp, Wolff Hendrik A, Rüschoff Josef, Ströbel Philipp, Grade Marian, Liersch Torsten, Beißbarth Tim, Ghadimi Michael B, Sax Ulrich, Gaedcke Jochen
Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
Department of Medical Statistics, University Medical Center, Goettingen, Germany.
Sci Rep. 2016 Oct 18;6:35589. doi: 10.1038/srep35589.
Translational research relies on high-quality biospecimens. In patients with rectal cancer treated preoperatively with radiochemotherapy tissue based analyses are challenging. To assess quality challenges we analyzed tissue samples taken over the last years in a multicenter setting. We retrospectively evaluated overall 197 patients of the CAO/ARO/AIO-94- and 04-trial with locally advanced rectal cancer that were biopsied preoperatively at the University Medical Center Goettingen as well as in 10 cooperating hospitals in Germany. The cellular content of tumor, mucosa, stroma, necrosis and the amount of isolated DNA and RNA as well as the RNA integrity number (RIN) as quality parameters were evaluated. A high RNA yield (p = 2.75e-07) and the content of tumor (p = 0.004) is significantly associated to high RIN-values, whereas a high content of mucosa (p = 0.07) shows a trend and a high amount of necrosis (p = 0.01) is significantly associated with RNA of poor quality. Correlating biopsies from Goettingen and the cooperating centers showed comparable tumor content results. By taking small sized biopsies we could assess a clear correlation between a good RNA quality and a high amount of RNA and tumor cells. These results also indicate that specimens collected at different centers are of comparable quality.
转化研究依赖于高质量的生物样本。对于术前接受放化疗的直肠癌患者，基于组织的分析具有挑战性。为了评估质量挑战，我们在多中心环境中分析了过去几年采集的组织样本。我们回顾性评估了CAO/ARO/AIO-94和04试验中总共197例局部晚期直肠癌患者，这些患者在哥廷根大学医学中心以及德国10家合作医院接受了术前活检。评估了肿瘤、黏膜、基质、坏死的细胞含量以及分离的DNA和RNA的量，以及作为质量参数的RNA完整性数值（RIN）。高RNA产量（p = 2.75e-07）和肿瘤含量（p = 0.004）与高RIN值显著相关，而高黏膜含量（p = 0.07）呈现出一种趋势，高坏死量（p = 0.01）与质量较差的RNA显著相关。对哥廷根和合作中心的相关活检进行比较，结果显示肿瘤含量结果具有可比性。通过采集小尺寸活检样本，我们能够评估良好的RNA质量与高RNA量和肿瘤细胞之间的明确相关性。这些结果还表明，在不同中心采集的样本质量具有可比性。
