Cengiz Mujgan, Alansal Nurnisa Oya, Tuncdemir Matem, Tanriverdi Gamze, Bayoglu Burcu
Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
Department of Histology and Embryology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
Indian J Pharmacol. 2016 Jul-Aug;48(4):407-411. doi: 10.4103/0253-7613.186213.
The aim of this study is to investigate the possible protective effects of melatonin and caffeic acid phenethyl ester (CAPE) on potassium dichromate (K CrO)-induced nephrotoxicity and genotoxicity.
A total of 40 Wistar albino rats were divided into five groups: control, KCrO(KCrO15 mg/kg, one dose, i.p.), KCrO + melatonin, KCrO + CAPE, and KCrO + melatonin + CAPE. Urine and blood samples were collected from rats before scarification. One kidney was collected for histopathological studies, and the other was stored at -80°C for further determination of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST), and glutathione reductase (GR) levels with spectrophotometric method. Comet assay was used to evaluate the genotoxicity.
We observed a significant amelioration in genotoxicity by melatonin and simultaneous melatonin + CAPE treatment compared to KCrO group (, < 0.05). SOD, CAT, GSH, GST, and MDA levels did not change when compared with controls. When KCrO applied group was treated with melatonin and CAPE, neither melatonin nor CAPE made any changes in kidney GSH, GST, SOD, and MDA levels ( > 0.05). We noted that treatment with CAPE and melatonin + CAPE together caused a significant decrease in renal tissue damage, an upregulation in the kidney CAT levels ( < 0.05) and a slight healing at GR levels when compared with the KCrO group.
Our results revealed, CAPE and melatonin may have protective effects on KCrO induced nephrotoxicity and cellular damage in rats.
本研究旨在探讨褪黑素和咖啡酸苯乙酯（CAPE）对重铬酸钾（K₂Cr₂O₇）诱导的肾毒性和遗传毒性的可能保护作用。
将40只Wistar白化大鼠分为五组：对照组、K₂Cr₂O₇组（K₂Cr₂O₇ 15 mg/kg，单次腹腔注射）、K₂Cr₂O₇ + 褪黑素组、K₂Cr₂O₇ + CAPE组以及K₂Cr₂O₇ + 褪黑素 + CAPE组。在处死大鼠前采集尿液和血液样本。取一侧肾脏进行组织病理学研究，另一侧肾脏保存在-80°C用于采用分光光度法进一步测定过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽S-转移酶（GST）和谷胱甘肽还原酶（GR）水平。采用彗星试验评估遗传毒性。
与K₂Cr₂O₇组相比，我们观察到褪黑素以及同时使用褪黑素 + CAPE治疗可显著改善遗传毒性（P < 0.05）。与对照组相比，SOD、CAT、GSH、GST和MDA水平无变化。当用褪黑素和CAPE处理K₂Cr₂O₇应用组时，褪黑素和CAPE均未使肾脏GSH、GST、SOD和MDA水平发生任何变化（P > 0.05）。我们注意到，与K₂Cr₂O₇组相比，CAPE以及褪黑素 + CAPE共同治疗可导致肾组织损伤显著减轻，肾脏CAT水平上调（P < 0.05），GR水平略有恢复。
我们的结果表明，CAPE和褪黑素可能对K₂Cr₂O₇诱导的大鼠肾毒性和细胞损伤具有保护作用。
