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理化学研究所生物资源中心生殖工程技术的发展。

Development of reproductive engineering techniques at the RIKEN BioResource Center.

作者信息

Ogura Atsuo

机构信息

RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.

出版信息

Exp Anim. 2017 Jan 27;66(1):1-16. doi: 10.1538/expanim.16-0074. Epub 2016 Oct 19.

DOI:10.1538/expanim.16-0074
PMID:27760894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5300997/
Abstract

Reproductive engineering techniques are essential for assisted reproduction of animals and generation of genetically modified animals. They may also provide invaluable research models for understanding the mechanisms involved in the developmental and reproductive processes. At the RIKEN BioResource Center (BRC), I have sought to develop new reproductive engineering techniques, especially those related to cryopreservation, microinsemination (sperm injection), nuclear transfer, and generation of new stem cell lines and animals, hoping that they will support the present and future projects at BRC. I also want to combine our techniques with genetic and biochemical analyses to solve important biological questions. We expect that this strategy makes our research more unique and refined by providing deeper insights into the mechanisms that govern the reproductive and developmental systems in mammals. To make this strategy more effective, it is critical to work with experts in different scientific fields. I have enjoyed collaborations with about 100 world-recognized laboratories, and all our collaborations have been successful and fruitful. This review summarizes development of reproductive engineering techniques at BRC during these 15 years.

摘要

生殖工程技术对于动物的辅助生殖和转基因动物的培育至关重要。它们还可能为理解发育和生殖过程中所涉及的机制提供极其宝贵的研究模型。在日本理化学研究所生物资源中心（BRC），我一直致力于开发新的生殖工程技术，特别是那些与冷冻保存、显微授精（精子注射）、核移植以及新干细胞系和动物的培育相关的技术，希望这些技术能够支持BRC目前和未来的项目。我还想将我们的技术与遗传和生化分析相结合，以解决重要的生物学问题。我们期望这一策略通过更深入地洞察哺乳动物生殖和发育系统的调控机制，使我们的研究更具独特性和精确性。为了使这一策略更有效，与不同科学领域的专家合作至关重要。我有幸与约100个世界知名实验室开展了合作，我们所有的合作都很成功且富有成果。这篇综述总结了BRC在这15年中生殖工程技术的发展情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/1bb6f01f8585/expanim-66-001-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/b0b441a91937/expanim-66-001-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/741c76fb402e/expanim-66-001-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/1bb6f01f8585/expanim-66-001-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/b0b441a91937/expanim-66-001-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/b2dc8c72f0ef/expanim-66-001-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/0b27b35343ae/expanim-66-001-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/e29805321ccd/expanim-66-001-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/c4fae434b46a/expanim-66-001-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1715/5300997/f20a480d5133/expanim-66-001-g007.jpg
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