Ziogas Vasileios, Tanou Georgia, Belghazi Maya, Diamantidis Grigorios, Molassiotis Athanassios
Faculty of Agriculture, Aristotle University of Thessaloniki, University Campus, 54124, Thessaloniki, Greece.
CNRS, UMR 7286, Plate-Forme de Recherche en Neurosciences PFRN, 13015, Marseille, France.
Plant Cell Rep. 2017 May;36(5):787-789. doi: 10.1007/s00299-016-2063-2. Epub 2016 Oct 19.
BABA or GABA induces salinity acclimation during citrus seeds germination via alternation of specific proteins (e.g., citrin). The impact of four elicitors, namely hydrogen peroxide (HO), β-amino butyric acid (BABA), γ-amino butyric acid (GABA) and hydrogen sulfide (HS) donor, sodium hydrosulfide (NaHS), in citrus seed germination under salinity (150 mM NaCl) was tested. The germination potential was adversely affected by NaCl-alone treatment. Pretreatment with HO or the NaHS-HS donor prior to salinity had no significant effect in germination process, however, BABA and GABA substantially improved seed acclimation to salinity, as evidenced by increased germination percentage and radicle length. Total soluble proteins of radicle and cotyledons were separated by 1DE SDS-PAGE and proteins zones were analyzed by mass spectrometry. In total, 27 and 3 proteins were identified in radicle and cotyledons, respectively. The identified proteins mainly include redox-regulated enzymes (i.e., glutathione S-transferase, dehydroascorbate reductase, Mn-superoxide dismutase, glutathione peroxidase), energy-related proteins (i.e., isocitrate lyase, malate synthase, pyruvate decarboxylase), stress proteins (i.e., stress-related protein, miraculin, thaumatin, disulfide isomerase), storage proteins (i.e., vicilin, Pis v 1 allergen 2S albumin) and transcriptional regulators (i.e., MarR family transcriptional regulator, MADS544 protein). Pretreatments with BABA or GABA altered the accumulation of protein zones exclusively corresponding to citrin, indicating that this protein may serve as a marker for salinity acclimation in citrus seeds.
BABA或GABA通过特定蛋白质（如柠檬素）的变化诱导柑橘种子萌发过程中的盐适应性。测试了四种诱导剂，即过氧化氢（HO）、β-氨基丁酸（BABA）、γ-氨基丁酸（GABA）和硫化氢（HS）供体硫氢化钠（NaHS）对盐胁迫（150 mM NaCl）下柑橘种子萌发的影响。单独用NaCl处理对发芽势有不利影响。在盐胁迫前用HO或NaHS-HS供体预处理对萌发过程没有显著影响，然而，BABA和GABA显著改善了种子对盐胁迫的适应性，发芽率和胚根长度增加证明了这一点。胚根和子叶的总可溶性蛋白通过1DE SDS-PAGE分离，蛋白质区域通过质谱分析。总共在胚根和子叶中分别鉴定出27种和3种蛋白质。鉴定出的蛋白质主要包括氧化还原调节酶（即谷胱甘肽S-转移酶、脱氢抗坏血酸还原酶、锰超氧化物歧化酶、谷胱甘肽过氧化物酶）、能量相关蛋白（即异柠檬酸裂解酶、苹果酸合酶、丙酮酸脱羧酶）、应激蛋白（即应激相关蛋白、奇异果甜蛋白、索马甜蛋白、二硫键异构酶）、贮藏蛋白（即豌豆球蛋白、豌豆过敏原2S白蛋白）和转录调节因子（即MarR家族转录调节因子、MADS544蛋白）。用BABA或GABA预处理改变了仅与柠檬素相对应的蛋白质区域的积累，表明该蛋白质可能作为柑橘种子盐适应性的标志物。
