Jisha K C, Puthur Jos T
Plant Physiology and Biochemistry Division, Department of Botany, University of Calicut, C.U. Campus P.O, Malappuram, Kerala, 673635, India.
Protoplasma. 2016 Mar;253(2):277-89. doi: 10.1007/s00709-015-0804-7. Epub 2015 Apr 3.
The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosynthetic and mitochondrial activities, and also modified the chlorophyll a fluorescence-related parameters. Moreover, BABA seed priming reduced malondialdehyde content in the seedlings and enhanced the accumulation of proline, total protein, total carbohydrate, nitrate reductase activity, and activities of antioxidant enzymes like guaiacol peroxidase and superoxide dismutase. Most of these positive features of BABA priming were predominantly exhibited when the plants were encountered with stress (NaCl/PEG). The BABA content in the BABA-treated green gram seeds and seedlings was also detected and quantified with high-performance thin layer chromatography (HPTLC), and it revealed that the priming effect of BABA initiated in seeds and further gets carried over to the seedlings. It was concluded that BABA seed priming improved the drought and salinity stress tolerance potential of all the three green gram varieties, and it was evident in the NaCl-tolerant variety Pusa Vishal as compared to Pusa Ratna (abiotic stress sensitive) and Pusa 9531(drought tolerant). Dual mode in cost effectiveness of BABA priming is evident from: (1) the positive features of priming are being exhibited more during the exposure of plants to stress, and (2) priming of seedlings can be carried out by BABA application to seeds at very low concentration and volume.
研究了β-氨基丁酸（BABA）对三种绿豆品种非生物胁迫耐受性的影响。BABA种子引发可缓解NaCl/聚乙二醇（PEG）胁迫下幼苗生长的降低，还能提高光合色素含量、光合和线粒体活性，并改变叶绿素a荧光相关参数。此外，BABA种子引发降低了幼苗中的丙二醛含量，增强了脯氨酸、总蛋白、总碳水化合物、硝酸还原酶活性以及愈创木酚过氧化物酶和超氧化物歧化酶等抗氧化酶的活性。当植物遭遇胁迫（NaCl/PEG）时，BABA引发的这些积极特性大多会显著表现出来。还采用高效薄层色谱法（HPTLC）检测并定量了经BABA处理的绿豆种子和幼苗中的BABA含量，结果表明BABA的引发作用始于种子，并进一步传递到幼苗中。得出的结论是，BABA种子引发提高了所有三个绿豆品种的干旱和盐胁迫耐受性，与普萨·拉特纳（对非生物胁迫敏感）和普萨9531（耐旱）相比，在耐NaCl品种普萨·维沙尔中这一点很明显。BABA引发在成本效益方面的双重模式体现在：（1）引发的积极特性在植物遭受胁迫时表现得更为明显，（2）通过以极低浓度和体积将BABA施用于种子即可对幼苗进行引发。
