Fu Guanhua, Ma Junfei, Wang Lihong, Yang Xin, Liu Jeruei, Zhao Xin
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China.
Institute of Biotechnology and Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Toxins (Basel). 2016 Oct 17;8(10):300. doi: 10.3390/toxins8100300.
Zearalenone (ZEA), an estrogenic mycotoxin, is mainly produced by fungi. In this study, CK1 isolated from soil with the capability of degrading ZEA was evaluated for its efficacy in reducing the adverse effects of ZEA in piglets. The gilts were fed one of the following three diets for 14 days: a basic diet for the control group; the basic diet supplemented with ZEA-contaminated basic diet for the treatment 1 (T1) group; and the basic diet supplemented with fermented ZEA-contaminated basic diet by CK1 for the treatment 2 (T2) group. The actual ZEA contents (analyzed) were 0, 1.20 ± 0.11, 0.47 ± 0.22 mg/kg for the control, T1, and T2 diets, respectively. The results showed that the T1 group had significantly increased the size of vulva and the relative weight of reproductive organs compared to the control group at the end of the trial. The T1 group significantly decreased the concentration of the luteinizing hormone (LH) compared with the control and T2 groups. Expression of ERβ was significantly up-regulated in the T2 group compared with the control. In addition, expression of ERβ was not different between the control and the T1 group. In summary, our results suggest that CK1 could detoxify ZEA in feed and reduce the adverse effects of ZEA in the gilts.
玉米赤霉烯酮（ZEA）是一种具有雌激素活性的霉菌毒素，主要由真菌产生。在本研究中，对从土壤中分离出的具有降解ZEA能力的CK1进行了评估，以确定其在减轻ZEA对仔猪不良影响方面的效果。将后备母猪分为三组，分别饲喂以下三种日粮14天：对照组为基础日粮；处理1（T1）组为添加了受ZEA污染的基础日粮；处理2（T2）组为添加了经CK1发酵的受ZEA污染的基础日粮。对照组、T1组和T2组日粮中实际ZEA含量（分析值）分别为0、1.20±0.11、0.47±0.22mg/kg。结果表明，在试验结束时，与对照组相比，T1组母猪的外阴大小和生殖器官相对重量显著增加。与对照组和T2组相比，T1组的促黄体生成素（LH）浓度显著降低。与对照组相比，T2组中雌激素受体β（ERβ）的表达显著上调。此外，对照组和T1组之间ERβ的表达没有差异。总之，我们的结果表明，CK1可以对饲料中的ZEA进行解毒，并减轻ZEA对后备母猪的不良影响。
