Qiao Ying, Mao Yong, Wang Jun, Chen Ruanni, Libing Zheng, Su Yong-Quan, Chen Jia, Zheng Wei-Qiang
State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co., Ltd, 352103, Fujian, China; College of Ocean and Earth Science, Xiamen University, 361005, China.
College of Ocean and Earth Science, Xiamen University, 361005, China.
Fish Shellfish Immunol. 2016 Dec;59:484-491. doi: 10.1016/j.fsi.2016.10.027. Epub 2016 Oct 17.
The white-spot disease caused by marine ciliate Cryptocryon irritans hindered the sustainable development of large yellow croaker Larimichthys crocea industry. Better understandings about the parasite-host interactions in the molecular level will facilitate the prevention of mass mortality of the L. crocea caused by white-spot disease. MicroRNAs (miRNAs) are a class of small RNA molecules about 20-22 nucleotides which post-transcriptionally regulated many protein-coding genes and involved in many biological processes, especially in host-pathogen responses. In this study, we identified known and novel miRNAs in the gill and liver of L. crocea challenged by C. irritans by high throughput sequencing using Solexa technology. The data were further studied to screen differentially expressed miRNAs, and predict their target genes. The differential expression (p < 0.05) between libraries was observed in 103 miRNAs in liver tissue, among which 65 and 38 were conserved and novel miRNAs, 67 and 36 were up- and down-regulated miRNAs. While in gill tissue, 122 significant differentially expressed miRNAs were identified, among which 83 and 39 were conserved and novel miRNAs, 79 and 43 were up- and down-regulated miRNAs. In addition, these differentially expressed miRNAs target a series of genes which involved in many important biological processes including immune response. Here via deep sequencing, we for the first time characterize L. crocea miRNAs in response to C. irritans challenge, the results should help for better understandings about the immune response of the L. crocea under C. irritans challenge.
由海洋纤毛虫刺激隐核虫引起的白点病阻碍了大黄鱼产业的可持续发展。在分子水平上更好地了解寄生虫与宿主的相互作用将有助于预防由白点病引起的大黄鱼大规模死亡。微小RNA（miRNA）是一类约20 - 22个核苷酸的小RNA分子，它们在转录后调控许多蛋白质编码基因，并参与许多生物学过程，尤其是在宿主 - 病原体反应中。在本研究中，我们使用Solexa技术通过高通量测序鉴定了受刺激隐核虫攻击的大黄鱼鳃和肝脏中的已知和新型miRNA。对数据进行进一步研究以筛选差异表达的miRNA，并预测它们的靶基因。在肝脏组织中，103个miRNA在文库间存在差异表达（p < 0.05），其中65个和38个分别为保守和新型miRNA，67个和36个分别为上调和下调的miRNA。而在鳃组织中，鉴定出122个显著差异表达的miRNA，其中83个和39个分别为保守和新型miRNA，79个和43个分别为上调和下调的miRNA。此外，这些差异表达的miRNA靶向一系列参与包括免疫反应在内的许多重要生物学过程的基因。通过深度测序，我们首次表征了受刺激隐核虫攻击的大黄鱼miRNA，结果应有助于更好地了解大黄鱼在刺激隐核虫攻击下的免疫反应。
