State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
Mar Biotechnol (NY). 2021 Apr;23(2):242-254. doi: 10.1007/s10126-021-10019-6. Epub 2021 Feb 20.
Large yellow croaker is an important marine culture species in China. Recently, the large yellow croaker industry is threatened by various disease problems, especially for the white spot disease, which is caused by parasite Cryptocaryon irritans. In the current study, we conducted a genome-wide association study (GWAS) for C. irritans resistance in two large yellow croaker populations (n = 264 and n = 480, respectively). We identified 15 QTL with explained genetic variance ranging from 1 to 8% in the two populations. One QTL on chromosome 23 was shared by the two populations, and three QTL had been reported in the previous study. We identified a lot of biological pathways associated with C. irritans resistance, such as hormone transport, response to bacterium, apoptotic process, acute inflammatory response to antigenic stimulus, and NF-kappa B signaling pathway. The genes casp8 and traf6 involved in regulatory network for apoptosis and inflammation were identified to be candidate genes for C. irritans resistance. Our results showed the complex polygenic architecture of resistance of large yellow croaker against C. irritans. These results would be helpful for the researches of the molecular mechanism of C. irritans resistance and genome-assisted breeding of large yellow croaker.
大黄鱼是中国重要的海水养殖品种。近年来，大黄鱼产业受到各种疾病问题的威胁，特别是白点病，由寄生虫刺激隐核虫引起。在本研究中，我们对两个大黄鱼群体（分别为 264 个和 480 个个体）的刺激隐核虫抗性进行了全基因组关联研究（GWAS）。我们在两个群体中鉴定了 15 个 QTL，其遗传方差解释率范围为 1%至 8%。23 号染色体上的一个 QTL在两个群体中共享，有三个 QTL 在先前的研究中已有报道。我们鉴定了与刺激隐核虫抗性相关的许多生物途径，如激素转运、对细菌的反应、凋亡过程、对抗原刺激的急性炎症反应和 NF-kappa B 信号通路。涉及凋亡和炎症调控网络的基因 casp8 和 traf6 被鉴定为刺激隐核虫抗性的候选基因。我们的研究结果显示大黄鱼对刺激隐核虫抗性的复杂多基因结构。这些结果将有助于研究刺激隐核虫抗性的分子机制和大黄鱼的基因组辅助育种。
