通过微生物培养和定量法对慢性暴露乳牙中作为根管冲洗剂的牙刷柄、蜂胶、次氯酸钠和生理盐水的抗菌活性进行比较评估。
Comparative evaluation of antimicrobial activity of miswak, propolis, sodium hypochlorite and saline as root canal irrigants by microbial culturing and quantification in chronically exposed primary teeth.
作者信息
Shingare Poonam, Chaugule Vishwas
机构信息
MDS, Senior Lecturer, Department of Pedodontics and Preventive Dentistry, M.G.M Dental College and Hospital, Kamothe, Navi Mumbai-400703, Maharashtra, India;
MDS, Professor and Head of Department of Pedodontics and Preventive Dentistry, Dr. D. Y. Patil Dental College and Hospital, Pimpri, Pune 411018, Maharashtra, India.
出版信息
Germs. 2011 Dec 24;1(1):12-21. doi: 10.11599/germs.2012.1004.
INTRODUCTION
One of the essential factors for successful root canal therapy is elimination of bacterial contaminants using an effective root canal chemical irrigant such as sodium hypochlorite which inherently possesses certain disadvantages like irritation to periapical tissues, staining of the instruments, burning of surrounding tissues etc. The aim of the study was to explore newer irrigant agents which would probably be as effective or more and at the same time less irritating to the tissues than sodium hypochlorite. Our search included two such agents, propolis and miswak.
METHODS
The study was performed on 40 infected primary teeth (20 male and 20 female patients). The subjects were divided into 4 groups of 10 children. Group 1 received 3% sodium hypochlorite as irrigating solution, group 2 received 12.5% alcoholic extract of miswak, group 3 received 11% alcoholic extract of propolis and group 4, 0.9% saline. We used the antimicrobial activity of sodium hypochlorite as gold standard for comparing the activities of both propolis and miswak. We collected pre- and post-irrigation samples using sterile paper points. Samples were cultured on tryptose soya agar at a temperature of 37(o)C for 24-48 hours. The colonies were counted with a digital colony counter. For the statistical analysis, we used the unpaired t test at level of significance 0.05 and the ANOVA test for analysis of variance.
RESULTS
The differences in pre- and post- irrigation values were calculated for each group, the greatest difference being seen in group 1 (95.549%) followed by group 2 (89.794%), group 3 (34.735%) and group 4 (28.087%). When comparing the results between groups, there was no statistically significant difference between groups 3 and 4.
CONCLUSIONS
The statistically analyzed results suggested that miswak could be a good natural substitute to sodium hypochlorite, while propolis showed results comparable to those of the negative control.
引言
根管治疗成功的关键因素之一是使用有效的根管化学冲洗剂消除细菌污染物，如次氯酸钠，但它本身存在一些缺点，如刺激根尖周组织、使器械染色、灼伤周围组织等。本研究的目的是探索新型冲洗剂，其可能与次氯酸钠一样有效或更有效，同时对组织的刺激性更小。我们的研究包括两种这样的药剂，蜂胶和阿拉伯树牙刷。
方法
对40颗感染的乳牙（20名男性和20名女性患者）进行研究。受试者被分为4组，每组10名儿童。第1组接受3%次氯酸钠作为冲洗液，第2组接受12.5%阿拉伯树牙刷酒精提取物，第3组接受11%蜂胶酒精提取物，第4组接受0.9%生理盐水。我们以次氯酸钠的抗菌活性作为金标准来比较蜂胶和阿拉伯树牙刷的活性。我们使用无菌纸尖收集冲洗前和冲洗后的样本。样本在胰蛋白胨大豆琼脂上于37℃培养24 - 48小时。用数字菌落计数器计数菌落。对于统计分析，我们使用显著性水平为0.05的非配对t检验和方差分析的ANOVA检验。
结果
计算每组冲洗前后数值的差异，第1组差异最大（95.549%），其次是第2组（89.794%）、第3组（34.735%）和第4组（28.087%）。比较各组结果时，第3组和第4组之间无统计学显著差异。
结论
统计分析结果表明，阿拉伯树牙刷可能是次氯酸钠的良好天然替代品，而蜂胶的结果与阴性对照相当。
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