Maclean Michelle, Anderson John G, MacGregor Scott J, White Tracy, Atreya Chintamani D
The Robertson Trust Laboratory for Electronic Sterilisation Technologies (ROLEST), Department of Electronic and Electrical Engineering, University of Strathclyde, 204 George Street, Glasgow, UK; Department of Biomedical Engineering, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow, UK.
The Robertson Trust Laboratory for Electronic Sterilisation Technologies (ROLEST), Department of Electronic and Electrical Engineering, University of Strathclyde, 204 George Street, Glasgow, UK.
J Blood Transfus. 2016;2016:2920514. doi: 10.1155/2016/2920514. Epub 2016 Sep 28.
Bacterial contamination of injectable stored biological fluids such as blood plasma and platelet concentrates preserved in plasma at room temperature is a major health risk. Current pathogen reduction technologies (PRT) rely on the use of chemicals and/or ultraviolet light, which affects product quality and can be associated with adverse events in recipients. 405 nm violet-blue light is antibacterial without the use of photosensitizers and can be applied at levels safe for human exposure, making it of potential interest for decontamination of biological fluids such as plasma. As a pilot study to test whether 405 nm light is capable of inactivating bacteria in biological fluids, rabbit plasma and human plasma were seeded with bacteria and treated with a 405 nm light emitting diode (LED) exposure system (patent pending). Inactivation was achieved in all tested samples, ranging from low volumes to prebagged plasma. 99.9% reduction of low density bacterial populations (≤10 CFU mL), selected to represent typical "natural" contamination levels, was achieved using doses of 144 Jcm. The penetrability of 405 nm light, permitting decontamination of prebagged plasma, and the nonrequirement for photosensitizing agents provide a new proof of concept in bacterial reduction in biological fluids, especially injectable fluids relevant to transfusion medicine.
室温下保存在血浆中的可注射储存生物液体（如血浆和浓缩血小板）的细菌污染是一个重大的健康风险。当前的病原体灭活技术（PRT）依赖于使用化学物质和/或紫外线，这会影响产品质量并可能与接受者的不良事件相关。405纳米的蓝紫光在不使用光敏剂的情况下具有抗菌作用，并且可以在对人体安全的暴露水平下应用，这使其对血浆等生物液体的去污具有潜在的吸引力。作为一项测试405纳米光是否能够灭活生物液体中细菌的初步研究，将细菌接种到兔血浆和人血浆中，并用405纳米发光二极管（LED）暴露系统（专利申请中）进行处理。在所有测试样品中都实现了灭活，从少量到预包装血浆均有测试。使用144 J/cm的剂量，对于选定代表典型“自然”污染水平的低密度细菌群体（≤10 CFU/mL）实现了99.9%的减少。405纳米光的穿透性使得预包装血浆能够被去污，并且不需要光敏剂，这为生物液体，特别是与输血医学相关的可注射液体中的细菌减少提供了一个新的概念验证。
