The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, China.
Front Cell Infect Microbiol. 2022 Feb 4;12:834015. doi: 10.3389/fcimb.2022.834015. eCollection 2022.
Over the past decade, has been recognized globally as a source of opportunistic infections. It is a yeast-like fungus, and its association as an uncommon pulmonary pathogen with cystic fibrosis patients has been previously reported. Immunocompromised patients are at the highest risk of infections. Therefore, to investigate the genetic basis for the pathogenicity of , we performed whole-genome sequencing and comparative genomic analysis of GMU1709 that was isolated from sputum specimens of a pneumonia patient receiving cardiac repair surgery. The assembly of Oxford Nanopore reads from the GMU1709 strain and its subsequent correction using Illumina paired-end reads yielded a high-quality complete genome with a genome size of 30.5 Mb in length, which comprised six chromosomes and one mitochondrion. Subsequently, 8,066 protein-coding genes were predicted based on multiple pieces of evidence, including transcriptomes. Phylogenomic analysis indicated that exhibited the closest evolutionary affinity to , and both the strains and the formerly ACCC 20271 strain occupied the same phylogenetic position. Further comparative analysis supported that the ACCC 20271 strain belonged to . Comparisons of three strains indicated that the differences between clinical and non-clinical strains in pathogenicity and drug resistance may be little or none. Based on the comparisons with strains of other species in the family, we identified potential key genetic factors associated with infection or pathogenicity. In addition, we also deduced that had great potential to inactivate some antibiotics (, tetracycline), which may affect the efficacy of these drugs in co-infection. In general, our analyses provide a better understanding of the classification and phylogeny of the family, uncover the underlying genetic basis of infections and associated drug resistance, and provide clues into potential targets for further research and the therapeutic intervention of infections.
在过去的十年中， 已被全球公认为机会性感染的来源。它是一种酵母样真菌，先前已有报道称其与囊性纤维化患者罕见的肺部病原体有关。免疫功能低下的患者感染 的风险最高。因此，为了研究 的致病性的遗传基础，我们对从接受心脏修复手术的肺炎患者的痰标本中分离出的 GMU1709 进行了全基因组测序和比较基因组分析。牛津纳米孔reads 的组装来自 GMU1709 菌株，随后使用 Illumina 配对末端 reads 进行校正，得到了一个高质量的完整基因组，其长度为 30.5Mb，包含六个染色体和一个线粒体。随后，根据包括转录组在内的多种证据预测了 8066 个蛋白质编码基因。系统发育基因组分析表明， 与 亲缘关系最近，并且 菌株和以前的 ACCC 20271 菌株都占据了相同的系统发育位置。进一步的比较分析支持 ACCC 20271 菌株属于 。对三个 菌株的比较表明，致病性和耐药性方面临床菌株与非临床菌株的差异可能很小或不存在。基于与 科其他物种菌株的比较，我们确定了与 感染或致病性相关的潜在关键遗传因素。此外，我们还推断 有很大的潜力使一些抗生素（如红霉素、四环素）失活，这可能会影响这些药物在合并感染中的疗效。总的来说，我们的分析更好地理解了 科的分类和系统发育，揭示了 感染和相关耐药性的潜在遗传基础，并为进一步研究和治疗干预感染提供了潜在目标的线索。
