Rolland J M, Nairn R C, Nind A P, Pihl E
J Natl Cancer Inst. 1984 Feb;72(2):267-73.
The double-zone fluorescein fluorescence polarization (FFP) "cancer" test was used to study 540 blood lymphocyte samples from 341 donors, of whom 158 had confirmed cancer: The other donors were noncancer patients, pregnant women, and normal individuals. The FFP response in cancer patients was the reverse of that in normal individuals, but an abnormal response was also obtained in some noncancer conditions, including the chronic inflammatory disorders--rheumatoid arthritis, cholecystitis, and diverticulitis--and in early pregnancy or pregnancy-induced hypertension. Thus the cancer discriminatory value of the test is limited. Examination of its biologic basis suggests that the positive FFP response in cancer and other conditions is due to altered immune status of blood lymphocytes, with associated change in cytoplasmic fluidity affecting the polarization of fluorescence. Incubation of normal blood lymphocytes with cyclic GMP induced an abnormal, cancer-like FFP response.
采用双区荧光素荧光偏振（FFP）“癌症”检测法对341名献血者的540份血液淋巴细胞样本进行了研究，其中158人确诊患有癌症，其他献血者为非癌症患者、孕妇和正常个体。癌症患者的FFP反应与正常个体相反，但在一些非癌症情况下也会出现异常反应，包括慢性炎症性疾病——类风湿性关节炎、胆囊炎和憩室炎——以及早孕或妊娠高血压。因此，该检测的癌症鉴别价值有限。对其生物学基础的研究表明，癌症和其他情况下FFP反应呈阳性是由于血液淋巴细胞免疫状态改变，细胞质流动性相关变化影响了荧光偏振。用环磷酸鸟苷孵育正常血液淋巴细胞会诱导出异常的、类似癌症的FFP反应。
