Hartmann W, Sackmann E, Eisert W G, Eisert R, Fliedner T M
Biomedicine. 1980 Dec;32(4):185-8.
Fluorescence polarization measurements were performed to detect PHA-stimulation of peripheral lymphocytes in an early state. Single cell fluorescence was measured by flow cytometry using an epi-illumination design to avoid the influence of background fluorescence and multiscattering. By these arrangements the accuracy in the determination of P, the degree of polarization, could be improved over the usual cell suspension measurements. Fluorescence polarization measurements were performed on density specified human lymphocytes after incubation with fluorescein diacetate with and without preincubation with phytohemagglutinin (PHA). A group of healthy donors had an average polarization of P = 0.17 before and P = 0.12 after PHA-stimulation, whereas a group of patients with malignant disease showed an average polarization of P = 0.14 before and after PHA-stimulation.
进行荧光偏振测量以检测外周淋巴细胞早期的PHA刺激。使用落射照明设计通过流式细胞术测量单细胞荧光，以避免背景荧光和多重散射的影响。通过这些设置，与通常的细胞悬液测量相比，偏振度P的测定准确性得以提高。在用荧光素二乙酸酯孵育后，对经密度分选的人淋巴细胞进行荧光偏振测量，其中一组在有或没有预先用植物血凝素（PHA）孵育的情况下进行。一组健康供体在PHA刺激前的平均偏振度P = 0.17，刺激后的平均偏振度P = 0.12，而一组恶性疾病患者在PHA刺激前后的平均偏振度均为P = 0.14。
