Forwood James M, Bubner Erin J, Landos Matt, Deveney Marty R, D'Antignana Trent
School of Biological Sciences, Lincoln Marine Science Centre, Flinders University, Port Lincoln, SA 5606, Australia.
Dis Aquat Organ. 2016 Oct 27;121(3):201-209. doi: 10.3354/dao03054.
Praziquantel (PZQ) is registered for oral use against Benedenia seriolae infecting Seriola spp. in Japan, but its bitter taste causes poor palatability. Incorporating PZQ in a moist pellet may help mask the flavor to improve intake. Altering delivery, however, may influence efficacy. We assessed the minimum effective concentrations of PZQ in moist pellets delivered by intubation for the monogeneans Zeuxapta seriolae and B. seriolae infecting yellowtail kingfish Seriola lalandi in flow-through tanks. The optimised dose was then evaluated in a sea-cage of S. lalandi to assess feed acceptance and efficacy. During intubation trials, efficacy was assessed as a percent reduction compared to control groups; in the field trial, efficacy was assessed by a percent reduction after treatment. PZQ delivered by intubation at 70 mg kg-1 body weight (BW) for 3 d was 99.7 and 81.7% effective against Z. seriolae and B. seriolae, respectively. Increasing the dose to 120 mg kg-1 BW for 3 d had a similar efficacy against Z. seriolae (98.4%) and increased efficacy against B. seriolae to 89.2%, but partial emesis of the medicated feed was sometimes noted. S. lalandi in a sea-cage at 17°C readily consumed PZQ administered daily in moist pellets at 70 mg kg-1 BW for 3 d (inclusion rate: 5.15 g kg-1), which significantly reduced Z. seriolae and B. seriolae abundance with 99.4 and 81.6% efficacy, respectively. Juvenile B. seriolae were common on the eyes of fish post-treatment which indicates a strategically timed repeat treatment is necessary.
吡喹酮（PZQ）在日本已注册用于口服治疗感染黄尾鰤属鱼类的鲈形贝尼登虫，但因其苦味导致适口性差。将PZQ掺入湿颗粒中可能有助于掩盖其味道以提高摄入量。然而，改变给药方式可能会影响疗效。我们评估了通过插管投喂湿颗粒时，PZQ对感染黄尾鰤（Seriola lalandi）的单殖吸虫——黄尾鰤泽氏吸虫（Zeuxapta seriolae）和鲈形贝尼登虫的最低有效浓度。然后在黄尾鰤的海水网箱中评估优化剂量，以评估饲料接受度和疗效。在插管试验中，与对照组相比，疗效以降低的百分比来评估；在现场试验中，疗效通过治疗后降低的百分比来评估。以70毫克/千克体重（BW）的剂量通过插管投喂PZQ，持续3天，对黄尾鰤泽氏吸虫和鲈形贝尼登虫的疗效分别为99.7%和81.7%。将剂量增加到120毫克/千克体重，持续3天，对黄尾鰤泽氏吸虫的疗效相似（98.4%），对鲈形贝尼登虫的疗效提高到89.2%，但有时会观察到药饵有部分呕吐现象。在17°C海水网箱中的黄尾鰤很乐意每天食用以70毫克/千克体重的剂量掺入湿颗粒中的PZQ，持续3天（添加率：5.15克/千克），这显著降低了黄尾鰤泽氏吸虫和鲈形贝尼登虫的丰度，疗效分别为99.4%和81.6%。治疗后，幼体鲈形贝尼登虫常见于鱼的眼睛上，这表明有必要进行策略性的定时重复治疗。
