Jensen Gitte S, Shah Bijal, Holtz Robert, Patel Ashok, Lo Donald C
NIS Labs, Klamath Falls, OR.
Department of Neurobiology, Center for Drug Discovery, Duke University Medical Center, Durham, NC.
Clin Cosmet Investig Dermatol. 2016 Oct 14;9:357-366. doi: 10.2147/CCID.S111999. eCollection 2016.
The aim of this study was to evaluate the effects of water-soluble egg membrane (WSEM) on wrinkle reduction in a clinical pilot study and to elucidate specific mechanisms of action using primary human immune and dermal cell-based bioassays.
To evaluate the effects of topical application of WSEM (8%) on human skin, an open-label 8-week study was performed involving 20 healthy females between the age of 45 years and 65 years. High-resolution photography and digital analysis were used to evaluate the wrinkle depth in the facial skin areas beside the eye (crow's feet). WSEM was tested for total antioxidant capacity and effects on the formation of reactive oxygen species by human polymorphonuclear cells. Human keratinocytes (HaCaT cells) were used for quantitative polymerase chain reaction analysis of the antioxidant response element genes , and . Evaluation of effects on human primary dermal fibroblasts in vitro included cellular viability and production of the matrix components collagen and elastin.
Topical use of a WSEM-containing facial cream for 8 weeks resulted in a significant reduction of wrinkle depth (<0.05). WSEM contained antioxidants and reduced the formation of reactive oxygen species by inflammatory cells in vitro. Despite lack of a quantifiable effect on Nrf2, WSEM induced the gene expression of downstream , and in human keratinocytes. Human dermal fibroblasts treated with WSEM produced more collagen and elastin than untreated cells or cells treated with dbcAMP control. The increase in collagen production was statistically significant (<0.05).
The topical use of WSEM on facial skin significantly reduced the wrinkle depth. The underlying mechanisms of this effect may be related to protection from free radical damage at the cellular level and induction of several antioxidant response elements, combined with stimulation of human dermal fibroblasts to secrete high levels of matrix components.
本研究旨在通过一项临床初步研究评估水溶性鸡蛋膜（WSEM）对减少皱纹的效果，并使用基于原代人免疫细胞和真皮细胞的生物测定法阐明其具体作用机制。
为评估局部应用8%WSEM对人体皮肤的影响，进行了一项为期8周的开放标签研究，纳入了20名年龄在45岁至65岁之间的健康女性。使用高分辨率摄影和数字分析评估眼部旁边面部皮肤区域（鱼尾纹）的皱纹深度。检测了WSEM的总抗氧化能力以及对人多形核细胞活性氧形成的影响。用人角质形成细胞（HaCaT细胞）对抗氧化反应元件基因、和进行定量聚合酶链反应分析。体外评估对人原代真皮成纤维细胞的影响包括细胞活力以及基质成分胶原蛋白和弹性蛋白的产生。
局部使用含WSEM的面霜8周可显著减少皱纹深度（<0.05）。WSEM含有抗氧化剂，并在体外减少了炎症细胞活性氧的形成。尽管对Nrf2缺乏可量化的影响，但WSEM诱导了人角质形成细胞中、和下游基因的表达。用WSEM处理的人真皮成纤维细胞比未处理的细胞或用dbcAMP对照处理的细胞产生更多的胶原蛋白和弹性蛋白。胶原蛋白产量的增加具有统计学意义（<0.05）。
在面部皮肤局部使用WSEM可显著减少皱纹深度。这种效果的潜在机制可能与在细胞水平上免受自由基损伤以及诱导多种抗氧化反应元件有关，同时还能刺激人真皮成纤维细胞分泌高水平的基质成分。
