• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于大肠杆菌起始因子3基因的特异性转导噬菌体。

Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.

作者信息

Springer M, Graffe M, Hennecke H

出版信息

Proc Natl Acad Sci U S A. 1977 Sep;74(9):3970-4. doi: 10.1073/pnas.74.9.3970.

DOI:10.1073/pnas.74.9.3970
PMID:333453
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431805/
Abstract

A previously isolated thermosensitive mutant [Springer, M., Graffe, M. & Grunberg-Manago, M. (6977) Mol. Gen. Genet. 151, 17-26] exhibits two defects in vitro, one in the initiation factor IF3 and the other in the L-phenylalanine: tRNA-Phe ligase (EC 6.1.1.20). Specialized lambda transducing phages that transduced this mutant to thermoresistance were selected. In vitro studies showed that the transductants had a normal IF3 activity. One of these transducing phages was shown to code for a protein synthesized under the control of Escherichia coli promoters, which has the same molecular weight as IF3. This protein crossreacts specifically with IF3 antisera and comigrates with pure IF3 in a two-dimensional gel system.

摘要

先前分离出的一个温度敏感突变体[施普林格，M.，格拉夫，M.和格伦贝格 - 马纳戈，M.（1967）《分子与普通遗传学》151卷，第17 - 26页]在体外表现出两种缺陷，一种是起始因子IF3方面的缺陷，另一种是L - 苯丙氨酸：苯丙氨酰 - tRNA连接酶（EC 6.1.1.20）方面的缺陷。筛选出了能将该突变体转导为耐热型的特异性λ转导噬菌体。体外研究表明，转导子具有正常的IF3活性。其中一种转导噬菌体被证明可编码一种在大肠杆菌启动子控制下合成的蛋白质，其分子量与IF3相同。这种蛋白质能与IF3抗血清发生特异性交叉反应，并且在双向凝胶系统中与纯IF3一同迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/a56ec3958534/pnas00031-0357-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/709023da9583/pnas00031-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/ada03e18aa03/pnas00031-0357-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/a56ec3958534/pnas00031-0357-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/709023da9583/pnas00031-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/ada03e18aa03/pnas00031-0357-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a687/431805/a56ec3958534/pnas00031-0357-b.jpg

相似文献

1
Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.用于大肠杆菌起始因子3基因的特异性转导噬菌体。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3970-4. doi: 10.1073/pnas.74.9.3970.
2
A specialized transducing lambda phage carrying the Escherichia coli genes for phenylalanyl-tRNA synthetase.一种携带大肠杆菌苯丙氨酰 - tRNA合成酶基因的特异性转导λ噬菌体。
Mol Gen Genet. 1977 Apr 29;152(3):205-10. doi: 10.1007/BF00268819.
3
Genetic organization of the E. coli chromosome around the structural gene for initiation factor IF3 (infC).大肠杆菌染色体围绕起始因子IF3（infC）结构基因的遗传组织。
Mol Gen Genet. 1979 Feb 1;169(3):337-43. doi: 10.1007/BF00382279.
4
Structural and transcriptional evidence for related thrS and infC expression.thrS和infC相关表达的结构和转录证据。
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6152-6. doi: 10.1073/pnas.80.20.6152.
5
Specialized lambda transducing bacteriophage which carries hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase.携带组氨酰 - 转移核糖核酸合成酶结构基因hisS的特异性λ转导噬菌体。
J Bacteriol. 1979 Aug;139(2):404-10. doi: 10.1128/jb.139.2.404-410.1979.
6
Characterization of an E. coli mutant with a thermolabile initiation factor IF3 activity.一种具有热不稳定起始因子IF3活性的大肠杆菌突变体的特性分析。
Mol Gen Genet. 1977 Feb 28;151(1):17-26. doi: 10.1007/BF00446908.
7
Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation in Escherichia coli.大肠杆菌中翻译起始因子IF3和核糖体蛋白L20受限的生理效应
Mol Gen Genet. 1996 Apr 10;250(6):705-14. doi: 10.1007/BF02172982.
8
Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.宿主在病毒装配中的作用：大肠杆菌groE基因的克隆及其蛋白质产物的鉴定。
Proc Natl Acad Sci U S A. 1978 Jan;75(1):136-9. doi: 10.1073/pnas.75.1.136.
9
Threonyl-transfer ribonucleic acid synthetase from Escherichia coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing lambda bacteriophage.来自大肠杆菌的苏氨酰转移核糖核酸合成酶：通过突变酶和特殊转导λ噬菌体对结构基因进行亚基结构和遗传分析。
J Bacteriol. 1977 Sep;131(3):943-50. doi: 10.1128/jb.131.3.943-950.1977.
10
Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation.从一组与翻译相关的基因中对大肠杆菌起始因子IF3的结构基因进行物理定位和克隆。
Gene. 1980 Oct;11(1-2):33-42. doi: 10.1016/0378-1119(80)90084-0.

引用本文的文献

1
In vitro characterisation of the MS2 RNA polymerase complex reveals host factors that modulate emesviral replicase activity.体外鉴定 MS2 RNA 聚合酶复合物揭示了调节emesviral 复制酶活性的宿主因子。
Commun Biol. 2022 Mar 25;5(1):264. doi: 10.1038/s42003-022-03178-2.
2
Translation initiation factor IF2 of the myxobacterium Stigmatella aurantiaca: presence of a single species with an unusual N-terminal sequence.粘细菌橙色标桩菌的翻译起始因子IF2：存在单一物种，其N端序列异常。
J Bacteriol. 1997 Apr;179(7):2348-55. doi: 10.1128/jb.179.7.2348-2355.1997.
3
Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth.

本文引用的文献

1
ISOLATION OF THE lambda PHAGE REPRESSOR.λ噬菌体阻遏物的分离
Proc Natl Acad Sci U S A. 1967 Feb;57(2):306-13. doi: 10.1073/pnas.57.2.306.
2
[System of repression insuring immunity in lysogenic bacteria].[确保溶源性细菌免疫性的抑制系统]
C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219-21.
3
Fractionation of translation initiation factor B (F3) into cistron-specific species.将翻译起始因子B（F3）分离为顺反子特异性物种。
核糖体循环因子（核糖体释放因子）对细菌生长至关重要。
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4249-53. doi: 10.1073/pnas.91.10.4249.
4
The Myxococcus xanthus dsg gene product performs functions of translation initiation factor IF3 in vivo.黄色黏球菌的dsg基因产物在体内发挥翻译起始因子IF3的功能。
J Bacteriol. 1994 Mar;176(5):1434-42. doi: 10.1128/jb.176.5.1434-1442.1994.
5
Transcription units around the gene for E. coli translation initiation factor IF3 (infC).大肠杆菌翻译起始因子IF3（infC）基因周围的转录单位。
Mol Gen Genet. 1982;186(2):247-52. doi: 10.1007/BF00331857.
6
Gene organization around the phenylalanyl-transfer ribonucleic acid synthetase locus in Escherichia coli.大肠杆菌中苯丙氨酰 - 转移核糖核酸合成酶基因座周围的基因组织。
J Bacteriol. 1981 Apr;146(1):269-74. doi: 10.1128/jb.146.1.269-274.1981.
7
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
8
Molecular cloning of Escherichia coli K-12 hexuronate system genes: exu region.大肠杆菌K-12己糖醛酸系统基因的分子克隆：exu区域
J Bacteriol. 1981 Jan;145(1):181-90. doi: 10.1128/jb.145.1.181-190.1981.
9
Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.包含大肠杆菌起始因子IF3结构基因的1.26千碱基DNA片段的序列：存在AUU起始密码子。
EMBO J. 1982;1(3):311-5. doi: 10.1002/j.1460-2075.1982.tb01166.x.
10
Escherichia coli phenylalanyl-tRNA synthetase operon: characterization of mutations isolated on multicopy plasmids.大肠杆菌苯丙氨酰 - tRNA合成酶操纵子：多拷贝质粒上分离出的突变的特征分析
J Bacteriol. 1982 Nov;152(2):650-60. doi: 10.1128/jb.152.2.650-660.1982.
FEBS Lett. 1970 Aug 17;9(4):213-217. doi: 10.1016/0014-5793(70)80358-1.
4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Messenger discriminating species of initiation factor F3.信使区分起始因子F3的种类。
Nat New Biol. 1971 Dec 22;234(51):236-9. doi: 10.1038/newbio234236a0.
6
Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins.二维聚丙烯酰胺凝胶电泳：一种用于核糖体蛋白的改进方法。
Anal Biochem. 1974 Jan;57(1):200-10. doi: 10.1016/0003-2697(74)90065-7.
7
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.平板凝胶上噬菌体T7早期RNA和蛋白质的分析。
J Mol Biol. 1973 Sep 15;79(2):237-48. doi: 10.1016/0022-2836(73)90003-x.
8
Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli.大肠杆菌丙氨酰 - tRNA合成酶突变体回复突变株中核糖体蛋白S20改变的遗传分析。
Mol Gen Genet. 1974;134(4):325-32. doi: 10.1007/BF00337467.
9
IF-3 requirements for initiation complex formation with synthetic messengers in E. coli system.大肠杆菌系统中IF-3与合成信使形成起始复合物的需求。
FEBS Lett. 1974 Sep 1;45(1):82-7. doi: 10.1016/0014-5793(74)80816-1.
10
Purification and properties of chain initiation factor 3 from T4-infected and uninfected Escherichia coli MRE 600. Stimulation of translation of synthetic and natural messengers.来自T4感染和未感染的大肠杆菌MRE 600的链起始因子3的纯化及性质。对合成和天然信使核糖核酸翻译的刺激作用。
J Biol Chem. 1974 Jun 25;249(12):3797-802.