Hennecke H, Springer M, Böck A
Mol Gen Genet. 1977 Apr 29;152(3):205-10. doi: 10.1007/BF00268819.
A lambda phage has been isolated which specifically transduces the Escherichia coli pheS and pheT genes coding for the alpha and beta subunits of the phenylalanyl-tRNA synthetase (PRS). This phage transduces with high frequency (i) several temperature-sensitive PRS mutants to thermoresistance and (ii) a p-fluorophenylalanine resistant PRS mutant to sensitivity against this amino-acid analog. The in vitro PRS activities of such lysogens suggest that the alpha and beta subunits coded by the transducing phage complement the mutant host PRS-subunits in vivo by means of formation of hybrid enzymes. The transducing lambda phages were also used to infect UV light irradiated cells. The SDS-gel electrophoretic analysis of the proteins synthesized in such cells revealed that the phage codes at least for four different E. coli proteins. Two proteins with molecular weights of 94,000 and 38,000 daltons cross-reacted with an anti PRS serum and were thus identified as the beta and alpha subunits of PRS, respectively. A third protein with a molecular weight of 22,000 daltons is identical with the ribosomal initiation factor IF3 (Springer et al., 1977b). The other protein (Mr 78,000) is still unidentified.
已分离出一种λ噬菌体，它能特异性转导编码苯丙氨酰 - tRNA合成酶（PRS）α和β亚基的大肠杆菌pheS和pheT基因。这种噬菌体高频转导：（i）几个温度敏感型PRS突变体使其具有耐热性，以及（ii）一个对对氟苯丙氨酸有抗性的PRS突变体使其对这种氨基酸类似物敏感。此类溶原菌的体外PRS活性表明，转导噬菌体编码的α和β亚基通过形成杂合酶在体内补充突变宿主的PRS亚基。转导性λ噬菌体也被用于感染紫外线照射的细胞。对此类细胞中合成的蛋白质进行的SDS - 凝胶电泳分析表明，该噬菌体至少编码四种不同的大肠杆菌蛋白质。两种分子量分别为94,000和38,000道尔顿的蛋白质与抗PRS血清发生交叉反应，因此分别被鉴定为PRS的β和α亚基。第三种分子量为22,000道尔顿的蛋白质与核糖体起始因子IF3相同（施普林格等人，1977b）。另一种蛋白质（分子量78,000）仍未鉴定出来。
