Comer M M
J Bacteriol. 1981 Apr;146(1):269-74. doi: 10.1128/jb.146.1.269-274.1981.
The organization of seven genes located at about 38 min on the genetic map of Escherichia coli was examined; these genes included pheS and pheT, which code for the alpha and beta subunits of phenylalanyl-transfer ribonucleic acid synthetase, and thrS, the structural gene for threonyl-transfer ribonucleic acid synthetase. Deletion mutants were isolated from an F-prime-containing merodiploid strain and were characterized genetically. Seventeen different kinds of deletions extending into pheS of pheT were identified. These deletions unambiguously defined the gene order as aroD pps himA pheT pheS thrS pfkB. Mutants with deletions covering either pheS or pheT, but not both, were analyzed further by assay of phenylalanyl-transfer ribonucleic acid synthetase. The phenotype of the mutants with a deletion from pfkB through pheS was anomalous; although the pheT gene was apparently still present, its product, the beta subunit, was much reduced in activity.
对位于大肠杆菌遗传图谱约38分钟处的七个基因的组织情况进行了研究；这些基因包括编码苯丙氨酰 - 转移核糖核酸合成酶α和β亚基的pheS和pheT，以及苏氨酰 - 转移核糖核酸合成酶的结构基因thrS。从含有F'因子的部分二倍体菌株中分离出缺失突变体并进行了遗传特征分析。鉴定出了17种延伸至pheS或pheT的不同类型的缺失。这些缺失明确确定了基因顺序为aroD pps himA pheT pheS thrS pfkB。通过测定苯丙氨酰 - 转移核糖核酸合成酶，进一步分析了缺失覆盖pheS或pheT但不同时覆盖两者的突变体。从pfkB到pheS缺失的突变体的表型异常；尽管pheT基因显然仍然存在，但其产物β亚基的活性大大降低。
