V.-N. Orekhovich Research Institute of Biomedical Chemistry, 10, Pogodinskaya St, Moscow, Russian Federation, 119121.
N.-N. Burdenko Research Institute of Neurosurgery, Moscow, Russia.
J Mol Neurosci. 2018 Mar;64(3):440-448. doi: 10.1007/s12031-018-1040-3. Epub 2018 Mar 5.
A comparative protein profile analysis of 17 blood plasma samples from patients with ischemia and 20 samples from healthy volunteers was carried out using ultra-high resolution mass spectrometry. The analysis of measurements was performed using the proteomics search engine OMSSA. Normalized spectrum abundance factor (NSAF) in the biological samples was assessed using SearchGUI. The findings of mass spectrometry analysis of the protein composition of blood plasma samples demonstrate that the depleted samples are quite similar in protein composition and relative abundance of proteins. By comparing them with the control samples, we have found a small group of 44 proteins characteristic of the blood plasma samples from patients with chronic cerebral ischemia. These proteins contribute to the processes of homeostasis maintenance, including innate immune response unfolding, the response of a body to stress, and contribution to the blood clotting cascade.
采用超高效分辨率质谱仪对 17 例缺血患者和 20 例健康志愿者的 17 份血浆样本进行了比较蛋白质谱分析。采用蛋白质组学搜索引擎 OMSSA 对测量结果进行了分析。使用 SearchGUI 评估了生物样本中的标准化谱丰度因子（NSAF）。对血浆样本蛋白质组成的质谱分析结果表明，耗竭样本在蛋白质组成和蛋白质相对丰度上非常相似。通过将它们与对照样本进行比较，我们发现了一组 44 种蛋白质，这些蛋白质是慢性脑缺血患者血浆样本的特征。这些蛋白质有助于维持内稳态的过程，包括先天免疫反应的展开、身体对压力的反应以及对凝血级联反应的贡献。
