Choi Hong Jin, Lee Jun Jae, Park Yeong Jun, Shin Jung-Woog, Sung Hak-Joon, Shin Ji Won, Wu Yanru, Kim Jeong Koo
Department of Biomedical Engineering, Inje University, Obang-Dong, Gimhae, Gyeongnam 621-749 Korea.
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212 USA.
Biomater Res. 2016 Oct 31;20:33. doi: 10.1186/s40824-016-0080-4. eCollection 2016.
Auxetic scaffolds (experimental) was fabricated by using poly(D, L-lactic-co-glycolic acid), 50:50, (PLGA) for effective bone cell proliferation with mechanical stimulation.
Negative Poisson's ratio in scaffold, 3-directional volumetric compression was applied during the scaffold fabrication at adequate temperature (60 °C). The pore size of scaffold ranged between 355 and 400 μm.
The porous morphology of the prepared auxetic scaffolds had shown partially concave and dent shapes in SEM image as expected. The lowest Poisson's ratios of experimental group was -0.07 at 60 °C/10 min. Compressive strength of experimental group was shown about 3.12 times higher than control group (conventional scaffold) in dry state at 25 °C. The compressive strengths of both groups were tended to be decreased dramatically in wet state compared to in dry state. However, compressive strengths of experimental group were higher 3.08 times and 1.88 times in EtOH/PBS (25 °C) and EtOH/PBS/DMEM (37 °C) than control group in wet state, respectively. Degradation rate of the scaffolds showed about 16 % weight loss in 5 weeks. In cell attachment test, experimental group showed 1.46 times higher cell proliferation than control group at 1-day with compressive stimulation. In 3-day culture, the experimental group showed 1.32 times higher than control group. However, there was no significant difference in cell proliferation in 5-day cultivation.
Overall, negative Poisson's ratio scaffolds with static mechanical stimulation could affect the cell proliferation at initial cultivation time.
采用50:50的聚（D，L-乳酸-共-乙醇酸）（PLGA）制备了具有负泊松比的支架（实验性），用于在机械刺激下实现有效的骨细胞增殖。
在适当温度（60°C）下制备支架时，对支架施加负泊松比和三向体积压缩。支架的孔径在355至400μm之间。
如预期的那样，制备的具有负泊松比的支架的多孔形态在扫描电子显微镜图像中显示出部分凹形和凹陷形状。实验组在60°C/10分钟时的最低泊松比为-0.07。在25°C干燥状态下，实验组的抗压强度比对照组（传统支架）高约3.12倍。与干燥状态相比，两组在潮湿状态下的抗压强度均显著降低。然而，在乙醇/磷酸盐缓冲液（25°C）和乙醇/磷酸盐缓冲液/杜氏改良 Eagle培养基（37°C）的潮湿状态下，实验组的抗压强度分别比对照组高3.08倍和1.88倍。支架的降解率在5周内显示出约16%的重量损失。在细胞附着试验中，实验组在1天的压缩刺激下细胞增殖比对照组高1.46倍。在3天培养中，实验组比对照组高1.32倍。然而，在5天培养中细胞增殖没有显著差异。
总体而言，具有静态机械刺激的负泊松比支架在初始培养时间可能会影响细胞增殖。
