Gunn Lynda, Finn Sarah, Hurley Daniel, Bai Li, Wall Ellen, Iversen Carol, Threlfall John E, Fanning Séamus
UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin Dublin, Ireland.
UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College DublinDublin, Ireland; Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk AssessmentBeijing, China.
Front Microbiol. 2016 Oct 21;7:1664. doi: 10.3389/fmicb.2016.01664. eCollection 2016.
Powdered infant formula (PIF) is not intended to be produced as a sterile product unless explicitly stated and on occasion may become contaminated during production with pathogens such as . This retrospective study focused on two historically reported salmonellosis outbreaks associated with PIF from the United Kingdom and France, in 1985 and 1996/1997. In this paper, the molecular characterization of the two outbreaks associated serovars Anatum and Ealing is reported. Initially the isolates were analyzed using pulsed-field gel electrophoresis (PFGE), which revealed the clonal nature of the two outbreaks. Following from this two representative isolates, one from each serovar was selected for whole genome sequencing (WGS), wherein analysis focused on the pathogenicity islands. Furthermore, the ability of these isolates to survive the host intercellular environment was determined using an gentamicin protection assay. Results suggest a high level of genetic diversity that may have contributed to survival and virulence of isolates from these outbreaks.
除非有明确说明，婴儿配方奶粉（PIF）并非无菌产品，且在生产过程中有时可能会被诸如……等病原体污染。这项回顾性研究聚焦于1985年以及1996/1997年分别在英国和法国发生的两起与婴儿配方奶粉相关的沙门氏菌病疫情。本文报道了与阿纳托姆血清型和伊灵血清型相关的这两起疫情的分子特征。最初，使用脉冲场凝胶电泳（PFGE）对分离株进行分析，结果显示这两起疫情具有克隆性质。在此基础上，从每个血清型中挑选出一个具有代表性的分离株进行全基因组测序（WGS），分析重点在于致病岛。此外，使用庆大霉素保护试验来确定这些分离株在宿主细胞间环境中的存活能力。结果表明存在高度的遗传多样性，这可能有助于这些疫情中分离株的存活和毒力。
