N Murugan, J Malathi, V Umashankar, H N Madhavan
Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, 6000 06, India; School of Chemical & Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613401, India.
Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, 6000 06, India.
Microbiol Res. 2016 Dec;193:140-149. doi: 10.1016/j.micres.2016.10.002. Epub 2016 Oct 19.
Multidrug-resistant (MDR) Pseudomonas aeruginosa VRFPA04, obtained from a keratitis patient was found to exhibit resistance to betalactam (Penicillins, cephalosporins, including carbapenems, except aztreonam), aminoglycosides, quinolone group of drugs and susceptible to colistin. The complete genome sequencing of the ocular isolate to measure and ascertain the degree of multidrug resistance in VRFPA04 strain resulted in 6,818,030bp (6.8Mb) genome sizes, which happen to be the third largest genome available in the Genbank to date. Two chromosomally integrated class I integrons carrying bla carbapenemase gene, multiple secretory systems consisting of types I-VI and VIII proteins and ocular virulence factors exo-T, Y, U and exotoxin A, a gene that inhibits protein synthesis which could have caused corneal cell death and Phytohormone auxin biosynthetic protein were detected in the genome of VRFPA04 Genome. In addition, 58 Regions of Genomic Plasticity (RGPs) regions, multiple phage genomes, genomic islands, CRISPR genes and RND family efflux pumps, such as MexCD-OprJ and MexEF-OprN and its regulators, MexT and MexR, were unraveled in VRFPA04. Thus, the current study reveals the virulence factors and resistome nature of an ocular isolate P aeruginosa VRFPA04 genome.
从一名角膜炎患者分离出的多重耐药（MDR）铜绿假单胞菌VRFPA04，被发现对β-内酰胺类（青霉素类、头孢菌素类，包括碳青霉烯类，氨曲南除外）、氨基糖苷类、喹诺酮类药物耐药，而对黏菌素敏感。对该眼部分离株进行全基因组测序以测量和确定VRFPA04菌株的多重耐药程度，结果显示基因组大小为6,818,030bp（6.8Mb），这恰好是迄今为止Genbank中第三大的可用基因组。在VRFPA04基因组中检测到两个携带bla碳青霉烯酶基因的染色体整合I类整合子、由I-VI型和VIII型蛋白组成的多个分泌系统以及眼部毒力因子外毒素T、Y、U和外毒素A，外毒素A是一种抑制蛋白质合成的基因，可能导致角膜细胞死亡，还检测到植物激素生长素生物合成蛋白。此外，在VRFPA04中还发现了58个基因组可塑性区域（RGP）、多个噬菌体基因组、基因组岛、CRISPR基因以及RND家族外排泵，如MexCD-OprJ和MexEF-OprN及其调节因子MexT和MexR。因此，本研究揭示了眼部分离株铜绿假单胞菌VRFPA04基因组的毒力因子和耐药组性质。
