Yu Sung-Hsun, Dubey Navneet Kumar, Li Wei-Shan, Liu Ming-Che, Chiang Han-Sun, Leu Sy-Jye, Shieh Ying-Hua, Tsai Feng-Chou, Deng Win-Ping
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan.
PLoS One. 2016 Nov 10;11(11):e0166342. doi: 10.1371/journal.pone.0166342. eCollection 2016.
Diabetic nephropathy is derived from long-term effects of high blood glucose on kidney function in type 2 diabetic patients. Several antidiabetic drugs and herbal medications have failed to prevent episodes of DN. Hence, this study aimed to further investigate the renal injury-reducing effect of antidiabetic CmNo1, a novel combination of powders of fruiting bodies and mycelia of Cordyceps militaris. After being administered with streptozotocin-nicotinamide and high-fat-diet, the diabetic nephropathy mouse model displayed elevated blood glucose and renal dysfunction markers including serum creatinine and kidney-to-body weight ratio. These elevated markers were significantly mitigated following 8 weeks CmNo1 treatment. Moreover, the chronic hyperglycemia-induced pathological alteration in renal tissue were also ameliorated. Besides, immunohistochemical study demonstrated a substantial reduction in elevated levels of carboxymethyl lysine, an advanced glycation end product. Elevated collagenous deposition in DN group was also attenuated through CmNo1 administration. Moreover, the enhanced levels of transforming growth factor-β1, a fibrosis-inducing protein in glomerulus were also markedly dampened. Furthermore, auxiliary risk factors in DN like serum triglycerides and cholesterol were found to be increased but were decreased by CmNo1 treatment. Conclusively, the results suggests that CmNo1 exhibit potent and efficacious renoprotective action against hyperglycemia-induced DN.
糖尿病肾病源于2型糖尿病患者长期高血糖对肾功能的影响。几种抗糖尿病药物和草药未能预防糖尿病肾病的发作。因此，本研究旨在进一步研究抗糖尿病药物CmNo1（一种由蛹虫草子实体和菌丝体粉末组成的新型组合）对减轻肾脏损伤的作用。在用链脲佐菌素-烟酰胺和高脂饮食处理后，糖尿病肾病小鼠模型出现血糖升高以及包括血清肌酐和肾体比在内的肾功能障碍标志物升高。在接受8周的CmNo1治疗后，这些升高的标志物显著减轻。此外，慢性高血糖诱导的肾组织病理改变也得到改善。此外，免疫组化研究表明，晚期糖基化终产物羧甲基赖氨酸水平显著降低。通过给予CmNo1，糖尿病肾病组中升高的胶原沉积也得到减轻。此外，肾小球中诱导纤维化的蛋白转化生长因子-β1水平的升高也明显受到抑制。此外，糖尿病肾病的辅助危险因素如血清甘油三酯和胆固醇被发现升高，但通过CmNo1治疗而降低。总之，结果表明CmNo1对高血糖诱导的糖尿病肾病具有强大而有效的肾脏保护作用。
