Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, AB, Canada.
Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
Clin Chim Acta. 2017 Jan;464:37-43. doi: 10.1016/j.cca.2016.10.037. Epub 2016 Nov 9.
Proton nuclear magnetic resonance (H NMR, or NMR) spectroscopy and inductively coupled plasma-mass spectrometry (ICP-MS) are commonly used for metabolomics and metal analysis in urine samples. However, creatinine quantification by NMR for the purpose of normalization of urinary metals has not been validated. We assessed the validity of using NMR analysis for creatinine quantification in human urine samples in order to allow normalization of urinary metal concentrations.
NMR and ICP-MS techniques were used to measure metabolite and metal concentrations in urine samples from 10 healthy subjects. For metabolite analysis, two magnetic field strengths (600 and 700MHz) were utilized. In addition, creatinine concentrations were determined by using the Jaffe method.
Creatinine levels were strongly correlated (R=0.99) between NMR and Jaffe methods. The NMR spectra were deconvoluted with a target database containing 151 metabolites that are present in urine. A total of 50 metabolites showed good correlation (R=0.7-1.0) at 600 and 700MHz. Metal concentrations determined after NMR-measured creatinine normalization were comparable to previous reports.
NMR analysis provided robust urinary creatinine quantification, and was sufficient for normalization of urinary metal concentrations. We found that NMR-measured creatinine-normalized urinary metal concentrations in our control subjects were similar to general population levels in Canada and the United Kingdom.
质子磁共振（H NMR，或 NMR）光谱和电感耦合等离子体质谱（ICP-MS）常用于尿液样本的代谢组学和金属分析。然而，NMR 分析用于肌酐定量以实现尿液金属的归一化尚未得到验证。我们评估了在人类尿液样本中使用 NMR 分析进行肌酐定量的有效性，以允许归一化尿液金属浓度。
使用 NMR 和 ICP-MS 技术测量了 10 名健康受试者尿液样本中的代谢物和金属浓度。对于代谢物分析，使用了两种磁场强度（600 和 700MHz）。此外，还使用 Jaffe 法测定肌酐浓度。
NMR 和 Jaffe 方法之间的肌酐水平具有很强的相关性（R=0.99）。NMR 光谱用包含尿液中存在的 151 种代谢物的目标数据库进行解卷积。在 600 和 700MHz 下，共有 50 种代谢物具有良好的相关性（R=0.7-1.0）。NMR 测定肌酐归一化后的金属浓度与先前的报告相当。
NMR 分析提供了强大的尿液肌酐定量，足以实现尿液金属浓度的归一化。我们发现，我们的对照受试者中通过 NMR 测量肌酐归一化后的尿液金属浓度与加拿大和英国的一般人群水平相似。
