Hinke J A, Menard M R
Respir Physiol. 1978 Apr;33(1):31-40. doi: 10.1016/0034-5687(78)90081-6.
Intracellular pH in single muscle fibres from Balanus nubilus was determined, both by the weak acid distribution method (using 5,5-dimethyl-2,4-oxazolidine dione) and by means of a glass pH microelectrode, following prolonged equilibrations in normal, acid or alkaline Ringer solutions. In the DMO method [3H]inulin was combined with [14C]DMO so that an extracellular space could be calculated for each 'DMO space' on each fibre. Less than ideal uptake curves were obtained both for [14C]DMO and [3H]inulin, yet the calculated pHi compared favourably with the microelectrode pHi, especially when the DMO-pHi was calculated from [14C]DMO and [3H]inulin values obtained by extrapolating the 'plateaus' of the uptake curves to time zero. The Waddell and Butler equation was examined to determine why acceptable pHi values should be forthcoming from less than ideal uptake data.
在正常、酸性或碱性任氏溶液中长时间平衡后，采用弱酸分布法（使用5,5-二甲基-2,4-恶唑烷二酮）和玻璃pH微电极测定了来自茗荷儿（Balanus nubilus）的单根肌纤维的细胞内pH。在DMO法中，将[3H]菊粉与[14C]DMO结合，以便为每根纤维上的每个“DMO空间”计算细胞外空间。[14C]DMO和[3H]菊粉均获得了不太理想的摄取曲线，但计算得到的细胞内pH与微电极测定的细胞内pH相比具有优势，特别是当从摄取曲线的“平台期”外推至时间零时获得的[14C]DMO和[3H]菊粉值计算DMO-细胞内pH时。研究了沃德尔（Waddell）和巴特勒（Butler）方程，以确定为什么从不太理想的摄取数据中应能得到可接受的细胞内pH值。
