Burton R F
Respir Physiol. 1978 Apr;33(1):51-8. doi: 10.1016/0034-5687(78)90083-x.
In the quantification of buffering the distinct roles of bicarbonate and of other buffers must be taken into account. The determination of the total non-bicarbonate buffer value, betaa, in intact tissues is complicated by active pH regulation and by heterogeneity of cytoplasm with respect to betaa, while heterogeneity with respect to pH in vivo could cause errors in estimates made with homogenates. Available estimates of betaa are discussed, as are the individual contributions of proteins, dipeptides and phosphates. A high betaa is appropriate in cells which sometimes have high rates of glycolysis, or which buffer extracellular fluid, but non-protein buffer concentrations can be well below the limits imposed by osmolarity, perhaps because buffering can upset ionic gradients.
在缓冲的量化过程中，必须考虑碳酸氢盐和其他缓冲剂的不同作用。完整组织中总非碳酸氢盐缓冲值（βa）的测定因活性pH调节以及细胞质中βa的异质性而变得复杂，而体内pH的异质性可能导致用匀浆进行的估计出现误差。文中讨论了现有的βa估计值，以及蛋白质、二肽和磷酸盐的各自贡献。在有时具有高糖酵解速率或缓冲细胞外液的细胞中，较高的βa是合适的，但非蛋白质缓冲剂浓度可能远低于渗透压所施加的限度，这可能是因为缓冲会扰乱离子梯度。
