当定向进化遇上祖先酶复活。

When directed evolution met ancestral enzyme resurrection.

作者信息

Alcalde Miguel

机构信息

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049, Madrid, Spain.

出版信息

Microb Biotechnol. 2017 Jan;10(1):22-24. doi: 10.1111/1751-7915.12452. Epub 2016 Nov 11.

DOI:10.1111/1751-7915.12452

PMID:27863072

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5270717/

Abstract

The directed evolution of ancestral -resurrected- enzymes can give a new twist in protein engineering approaches towards more versatile and robust biocatalysts.

摘要

对祖先复活酶的定向进化可为蛋白质工程方法带来新的转机，以获得更通用、更强大的生物催化剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c68d/5270717/e5c0ab2257ec/MBT2-10-22-g001.jpg

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Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins.

Proteins. 2014 Jun;82(6):887-96. doi: 10.1002/prot.24575. Epub 2014 Apr 18.

Experimental evidence for the thermophilicity of ancestral life.

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PLoS One. 2012;7(7):e41175. doi: 10.1371/journal.pone.0041175. Epub 2012 Jul 31.

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当定向进化遇上祖先酶复活。

When directed evolution met ancestral enzyme resurrection.

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当定向进化遇上祖先酶复活。

When directed evolution met ancestral enzyme resurrection.

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机构信息

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