Galtier P, Paulin F, Eeckhoutte C, Larrieu G
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Food Chem Toxicol. 1989 Apr;27(4):215-20. doi: 10.1016/0278-6915(89)90158-0.
The effects of T-2 toxin and diacetoxyscirpenol on tissue drug-metabolizing enzymes in young male rats were compared. Mycotoxicoses were produced by daily oral administration of toxins at 1.0 mg/kg body weight for 1, 4 or 8 days. Many hepatic, renal and pulmonary oxidative and conjugative enzymes were measured in animals killed 24 hr following the last administration. The effects of the two trichothecene mycotoxins were generally similar. In liver the decrease in microsomal and cytosolic proteins paralleled the decline in total plasma proteins or the increase in plasma GOT activity. Hepatic microsomal cytochrome P-450 decreased in rats receiving trichothecenes for 8 days. This effect was more marked when aminopyrine, benzphetamine, ethylmorphine and ethoxycoumarin dealkylations or aniline and benzopyrene hydroxylations were measured. p-nitrophenol glucuronyltransferase activity was enhanced in animals receiving at least one administration of trichothecenes, whereas there was no change in conjugation to glutathione or acetate. In other tissues, there was no change in any renal enzymes whereas a significant rise in pulmonary monooxygenase was observed in T-2 toxin administered to rats for 4 or 8 days.
比较了T-2毒素和二醋酸雪腐镰刀菌烯醇对年轻雄性大鼠组织药物代谢酶的影响。通过每天以1.0毫克/千克体重口服毒素，持续1、4或8天来诱发霉菌毒素中毒。在最后一次给药后24小时处死的动物中，检测了许多肝脏、肾脏和肺脏的氧化酶和结合酶。两种单端孢霉烯族霉菌毒素的影响总体相似。在肝脏中，微粒体和胞质蛋白的减少与血浆总蛋白的下降或血浆谷草转氨酶活性的增加平行。接受单端孢霉烯族毒素处理8天的大鼠肝脏微粒体细胞色素P-450减少。当检测氨基比林、苄非他明、乙基吗啡和乙氧香豆素脱烷基作用或苯胺和苯并芘羟化作用时，这种影响更为明显。接受至少一次单端孢霉烯族毒素处理的动物中，对硝基苯酚葡糖醛酸基转移酶活性增强，而与谷胱甘肽或乙酸酯的结合没有变化。在其他组织中，任何肾脏酶均无变化，而在给大鼠施用T-2毒素4或8天的情况下，观察到肺单加氧酶显著升高。
