de Albuquerque Ricardo Diego Duarte Galhardo, Perini Jamila Alessandra, Machado Daniel Escorsim, Angeli-Gamba Thaís, Esteves Ricardo Dos Santos, Santos Marcelo Guerra, Oliveira Adriana Passos, Rocha Leandro
Programa de Pós Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil; Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, RJ, Brasil.
Laboratório de Pesquisas em Ciências Farmacêuticas, Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Pharmacogn Mag. 2016 Oct-Dec;12(48):288-294. doi: 10.4103/0973-1296.192206.
is an endemic species from Brazil. Eugenia genus has flavonoids as one of the remarkable chemical classes which are related to the improvement of the healing process.
To evaluate of wound healing activity of leaves and to identify and quantify its main flavonoids compounds.
Wound excision model in rats was used to verify the hydroethanolic and ethyl acetate extracts potential. The animals were divided in four groups of six and the samples were evaluated until the 15° day of treatment. Hydroxyproline dosage and histological staining with hematoxilin-eosin and Sirius Red were used to observe the tissue organization and quantify the collagen deposition, respectively. Chemical compounds of the ethyl acetate extract were identified by chromatographic techniques and mass spectrometry analysis and total flavonoids content was determined by spectrophotometric method. The antioxidant activity was determined by oxygen radical absorbing capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazylhydrate radical photometric (DPPH) assays.
The treated group with the ethyl acetate extract showed collagen deposition increase, higher levels of hidroxyproline, better tissue reorganization and complete remodeling of epidermis. Quercetin, kaempferol and hyperoside were identified as main compounds and flavonoids content value was 43% (w/w). The ORAC value of the ethyl acetate extract was 0.81± 0.05 mmol TE/g whereas the concentration to produce 50% reduction of the DPPH was 7.05± 0.09 μg/mL.
The data indicate a wound healing and antioxidant activities of . This study is the first report of flavonoids and wound healing activity of .
extract accelerates wound healing in skin rat model, probably due to its involvement with the collagen deposition increase, higher levels of hidroxyproline, dermal remodelling and potent antioxidant activity. Chemical standardization of the active wound healing extract was done. The total flavonoid content was 43% (w/w) and quercetin, kaempferol and hyperoside were identified as main compounds.
Wound excision model in rats showed the potential wound healing activity of by collagen deposition increase, higher levels of hidroxyproline, better tissue reorganization and complete remodeling of epidermis.Flavonoids are the main compounds of the endemic and quercetin, kaempferol and hyperoside were identified in ethyl acetate extract by TLC, HPLC-PDA and HRESI-MS analysis.The ethyl acetate extract of showed a potent antioxidant activity by ORAC and DPPH assays NC: Negative control, PC: Positive control, CH: Crude hydroethanolic extract, EA: Ethyl acetate extract, TE: Trolox equivalent, mg: Milligram, mM: Millimolar, mL: Milliliter, HPLC-PDA: High performance liquid chromatography with a photodiode array detector, HRESI-MS: High-resolution electrospray ionization mass spectrometry analysis, TLC: Thin layer chromatography, ORAC: Oxygen radical absorbance capacity, w/v: Weight per volume.
[该植物名称]是一种来自巴西的本土物种。番樱桃属含有黄酮类化合物，这是一类显著的化学物质，与促进愈合过程有关。
评估[该植物名称]叶的伤口愈合活性，并鉴定和定量其主要黄酮类化合物。
采用大鼠伤口切除模型来验证水乙醇提取物和乙酸乙酯提取物的潜力。将动物分为四组，每组六只，对样品进行评估直至治疗的第15天。分别采用羟脯氨酸定量以及苏木精-伊红和天狼星红组织学染色来观察组织结构并定量胶原蛋白沉积。通过色谱技术和质谱分析鉴定乙酸乙酯提取物中的化合物，并采用分光光度法测定总黄酮含量。通过氧自由基吸收能力（ORAC）和2,2-二苯基-1-苦基肼自由基光度法（DPPH）测定抗氧化活性。
用乙酸乙酯提取物处理的组显示胶原蛋白沉积增加、羟脯氨酸水平升高、组织重组更好且表皮完全重塑。鉴定出槲皮素、山奈酚和金丝桃苷为主要化合物，黄酮类化合物含量值为43%（w/w）。乙酸乙酯提取物的ORAC值为0.81±0.05 mmol TE/g，而使DPPH降低50%的浓度为7.05±0.09 μg/mL。
数据表明[该植物名称]具有伤口愈合和抗氧化活性。本研究是关于[该植物名称]黄酮类化合物及其伤口愈合活性的首次报道。
[该植物名称]提取物可加速大鼠皮肤模型中的伤口愈合，可能是由于其参与了胶原蛋白沉积增加、羟脯氨酸水平升高、真皮重塑以及强大的抗氧化活性。对具有活性的伤口愈合提取物进行了化学标准化。总黄酮含量为43%（w/w），鉴定出槲皮素、山奈酚和金丝桃苷为主要化合物。
大鼠伤口切除模型显示[该植物名称]通过增加胶原蛋白沉积、提高羟脯氨酸水平、更好地组织重组和表皮完全重塑具有潜在的伤口愈合活性。黄酮类化合物是[该植物名称]的主要化合物，通过薄层色谱（TLC）、高效液相色谱-光电二极管阵列检测器（HPLC-PDA）和高分辨率电喷雾电离质谱分析（HRESI-MS）在乙酸乙酯提取物中鉴定出了槲皮素、山奈酚和金丝桃苷。通过ORAC和DPPH测定，[该植物名称]的乙酸乙酯提取物显示出强大的抗氧化活性 NC：阴性对照，PC：阳性对照，CH：粗水乙醇提取物，EA：乙酸乙酯提取物，TE：Trolox当量，mg：毫克，mM：毫摩尔，mL：毫升，HPLC-PDA：配备光电二极管阵列检测器的高效液相色谱，HRESI-MS：高分辨率电喷雾电离质谱分析，TLC：薄层色谱，ORAC：氧自由基吸收能力，w/v：重量/体积
