Ogawa K, Miyaji H, Kato A, Kosen Y, Momose T, Yoshida T, Nishida E, Miyata S, Murakami S, Takita H, Fugetsu B, Sugaya T, Kawanami M
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
Support Section for Education and Research, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
J Periodontal Res. 2016 Dec;51(6):758-767. doi: 10.1111/jre.12352. Epub 2016 Jan 14.
Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (β-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects, on bioactivity, of a nano-β-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing.
Beta-tricalcium phosphate was pulverized into nanosize particles (84 nm) and was then dispersed. A nano-β-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nanosize β-TCP dispersion. Scaffolds were characterized using scanning electron microscopy, compressive testing, cell seeding and rat subcutaneous implant testing. Then, nano-β-TCP scaffold, nano-β-TCP scaffold loaded with FGF-2 and noncoated collagen scaffold were implanted into a dog one-wall infrabony defect model. Histological observations were made at 10 d and 4 wk postsurgery.
Scanning electron microscopy images show that TCP nanoparticles were attached to collagen fibers. The nano-β-TCP scaffold showed higher compressive strength and cytocompatibility compared with the noncoated collagen scaffold. Rat subcutaneous implant tests showed that the DNA contents of infiltrating cells in the nano-β-TCP scaffold and the FGF-2-loaded scaffold were approximately 2.8-fold and 3.7-fold greater, respectively, than in the collagen scaffold. Histological samples from the periodontal defect model showed about five-fold greater periodontal tissue repair following implantation of the nano-β-TCP scaffold loaded with FGF-2 compared with the collagen scaffold.
The β-TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano-β-TCP scaffolds containing FGF-2 are anticipated for use in periodontal tissue engineering.
纳米生物陶瓷正被用于生物医学应用研究。我们制备了一种由I型胶原蛋白和β-磷酸三钙（β-TCP）纳米颗粒组成的再生支架。成纤维细胞生长因子-2（FGF-2）是一种具有生物活性的信号分子，可刺激细胞增殖和伤口愈合。本研究考察了负载FGF-2的纳米β-TCP/胶原蛋白支架对生物活性的影响，尤其是对牙周组织伤口愈合的影响。
将β-磷酸三钙粉碎成纳米级颗粒（84纳米），然后进行分散。通过用纳米级β-TCP分散液涂覆胶原蛋白支架表面制备纳米β-TCP支架。使用扫描电子显微镜、压缩测试、细胞接种和大鼠皮下植入测试对支架进行表征。然后，将纳米β-TCP支架、负载FGF-2的纳米β-TCP支架和未涂层的胶原蛋白支架植入犬单壁骨下缺损模型。在术后10天和4周进行组织学观察。
扫描电子显微镜图像显示TCP纳米颗粒附着在胶原纤维上。与未涂层的胶原蛋白支架相比，纳米β-TCP支架表现出更高的抗压强度和细胞相容性。大鼠皮下植入测试表明，纳米β-TCP支架和负载FGF-2的支架中浸润细胞的DNA含量分别比胶原蛋白支架高约2.8倍和3.7倍。牙周缺损模型的组织学样本显示，与胶原蛋白支架相比，植入负载FGF-2的纳米β-TCP支架后牙周组织修复程度约高五倍。
β-TCP纳米颗粒涂层显著提高了胶原蛋白支架的生物活性。含有FGF-2的纳米β-TCP支架有望用于牙周组织工程。
