Ogawa K, Matsuda S, Seto A
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Leuk Res. 1989;13(5):399-406. doi: 10.1016/0145-2126(89)90080-5.
An HTLV-I-transformed T-cell line from a (B/J x Chbb:HM)F1 rabbit was intravenously inoculated at a dose of 1 x 10(8) cells into B/J and Chbb:HM adult rabbits. All B/J rabbits died or became moribund 6-13 days later, whereas all Chbb:HM rabbits survived without disease symptoms. Marked leukocytosis was observed in Chbb:HM rabbits but not in B/J rabbits, the leukocyte counts in the peripheral blood of the former being 10-30 times the normal level 10 days post-inoculation with most cells being of normal lymphoid appearance. Pathological examination of dead B/J animals revealed leukemic infiltration of abnormal lymphocytes in major organs, the infiltrate being composed of medium-sized lymphoid cells with pan T-cell marker, convoluted vesicular nuclei and frequent mitotic figures. These results indicate that allogeneic transfer of HTLV-I-transformed cells can induce leukemic infiltration under appropriate conditions. Similar leukemic infiltration was observed in major organs of random-bred rabbits 72 h after a similar inoculation of the same cell line. Such adult T-cell leukemia-like disease induced in random-bred rabbits may be useful in studying the mechanism and the prevention of leukemic infiltration.
将来自一只（B/J×Chbb:HM）F1兔的人嗜T淋巴细胞病毒I型（HTLV-I）转化的T细胞系，以1×10⁸个细胞的剂量静脉接种到B/J和Chbb:HM成年兔体内。所有B/J兔在6 - 13天后死亡或濒死，而所有Chbb:HM兔存活且无疾病症状。在Chbb:HM兔中观察到明显的白细胞增多，而B/J兔中未观察到，前者接种后10天外周血白细胞计数是正常水平的10 - 30倍，大多数细胞具有正常淋巴细胞外观。对死亡的B/J动物进行病理检查发现，主要器官有异常淋巴细胞的白血病浸润，浸润细胞由具有全T细胞标志物、卷曲泡状核和频繁有丝分裂象的中等大小淋巴细胞组成。这些结果表明，在适当条件下，HTLV-I转化细胞的同种异体转移可诱导白血病浸润。在随机繁殖的兔中以相同方式接种相同细胞系72小时后，在其主要器官中观察到类似的白血病浸润。在随机繁殖的兔中诱导的这种成人T细胞白血病样疾病可能有助于研究白血病浸润的机制和预防。
