Seto A, Kawanishi M, Matsuda S, Ogawa K, Miyoshi I
Department of Microbiology, Faculty of Medicine, Kyoto University.
Jpn J Cancer Res. 1988 Mar;79(3):335-41. doi: 10.1111/j.1349-7006.1988.tb01596.x.
An HTLV-I-transformed T cell line, obtained from the peripheral blood of a virus-infected (B/J X Chbb:HM) F1 rabbit, was able to kill syngeneic newborn rabbits within 7 days, when inoculated intraperitoneally at a dose of 1 X 10(8) cells. Inoculation of 1 X 10(7) cells killed or rendered moribund 50% of inoculated animals, while surviving animals exhibited cell-mediated cytotoxic activities against the transformed cells. The peripheral blood leukocyte counts increased in all surviving animals, in association with appearance of abnormal lymphocytes with convoluted or lobulated nuclei. Pathological examination of animals that died one week post-inoculation revealed no tumors in the abdominal cavity, but accumulation of ascites containing abnormal lymphocytes. Histological examination showed leukemic infiltration in the liver, lungs, spleen and mesenteric lymph nodes. The same cell line was also able to kill syngeneic adult rabbits in 8-10 days when inoculated intravenously, but not intraperitoneally, at a dose of 1 X 10(8) cells. Leukemic infiltration was observed in the major organs of these animals. Adult animals which were already virus carriers were resistant to this lethal inoculation. This rabbit ATL-like disease may prove to be useful as an experimental model for acute adult T cell leukemia.
从病毒感染的（B/J×Chbb:HM）F1兔外周血中获得的一株人嗜T淋巴细胞病毒I型（HTLV-I）转化的T细胞系，当以1×10⁸个细胞的剂量腹腔接种时，能够在7天内杀死同基因新生兔。接种1×10⁷个细胞可导致50%的接种动物死亡或濒死，而存活的动物表现出针对转化细胞的细胞介导细胞毒性活性。所有存活动物的外周血白细胞计数均增加，同时出现核呈卷曲或分叶状的异常淋巴细胞。对接种后一周死亡的动物进行病理检查发现腹腔内无肿瘤，但有含有异常淋巴细胞的腹水积聚。组织学检查显示肝脏、肺、脾脏和肠系膜淋巴结有白血病浸润。当以1×10⁸个细胞的剂量静脉注射而非腹腔注射时，同一细胞系也能够在8至10天内杀死同基因成年兔。在这些动物的主要器官中观察到白血病浸润。已经是病毒携带者的成年动物对这种致死性接种具有抗性。这种兔类成人T细胞白血病样疾病可能被证明是急性成人T细胞白血病的有用实验模型。
