Isono T, Isegawa Y, Seto A
Department of Microbiology, Shiga University of Medical Science, Japan.
J Cancer Res Clin Oncol. 1996;122(8):458-64. doi: 10.1007/BF01187157.
Peripheral blood lymphocytes (PBL) from rabbits of the Chbb:HM strain proliferated in coculture with an X-ray-irradiated HTLV-I-transformed leukemogenic cell line of (B/J x Chbb:HM) F1 origin, whereas PBL from rabbits of the B/J and F1 strains hardly proliferated at all in co-culture with the same cell line. A proviral HTLV-I genome was detected in high-molecular-mass DNA from these proliferating cells. An analysis of T cell receptor V beta expression revealed that these lymphocytes were of restricted V beta subfamilies, suggesting that the preferential stimulation and transformation of lymphocytes occurred in this co-culture. Staphylococcal enterotoxins similarly stimulated lymphocytes and the proliferated lymphocytes were mostly of distinct V beta subfamilies depending on stimulator enterotoxins. These results suggested that the leukemogenic cell line possesses an antigen that preferentially stimulates lymphocytes of restricted V beta subfamilies.
Chbb:HM品系兔的外周血淋巴细胞（PBL）与经X射线照射的源自（B/J×Chbb:HM）F1的HTLV-I转化的致白血病细胞系共培养时会增殖，而B/J品系和F1品系兔的PBL与同一细胞系共培养时几乎完全不增殖。在这些增殖细胞的高分子量DNA中检测到前病毒HTLV-I基因组。对T细胞受体Vβ表达的分析表明，这些淋巴细胞属于受限的Vβ亚家族，这表明淋巴细胞的优先刺激和转化发生在这种共培养中。葡萄球菌肠毒素同样能刺激淋巴细胞，增殖的淋巴细胞大多根据刺激肠毒素属于不同的Vβ亚家族。这些结果表明，该致白血病细胞系拥有一种能优先刺激受限Vβ亚家族淋巴细胞的抗原。
