Seto A, Kawanishi M, Matsuda S, Ogawa K, Eguchi T, Miyoshi I
Department of Microbiology, Faculty of Medicine, Kyoto University.
Jpn J Cancer Res. 1987 Nov;78(11):1150-5.
Two HTLV-I-carrying T cell lines were prepared from peripheral lymphocytes of a virus-infected (B/J X Chbb:HM) F1 rabbit, and these cells were inoculated intraperitoneally into 5 newborn F1 rabbits. These animals were killed 3-5 weeks later. Their leukocyte counts were higher than those in normal control animals, with abnormal lymphocytes amounting to 3-5% of total leukocytes. Histological examination showed leukemic infiltration in liver, spleen, lung and kidneys of all these animals. The peripheral lymphocytes at first lacked HTLV-I antigens, but became antigen-positive after in vitro culture. Southern blot analysis of these cells revealed HTLV-I integration patterns different from those of the inoculated cells.
从一只感染病毒的（B/J×Chbb:HM）F1代兔的外周淋巴细胞中制备了两株携带人类嗜T淋巴细胞病毒I型（HTLV-I）的T细胞系，并将这些细胞腹腔内接种到5只新生F1代兔体内。3至5周后处死这些动物。它们的白细胞计数高于正常对照动物，异常淋巴细胞占白细胞总数的3%至5%。组织学检查显示，所有这些动物的肝脏、脾脏、肺和肾脏均有白血病浸润。外周淋巴细胞最初缺乏HTLV-I抗原，但在体外培养后变为抗原阳性。对这些细胞进行的Southern印迹分析显示，HTLV-I的整合模式与接种细胞的不同。
