脂联素在皮肤恶性黑色素瘤中的表达。

Adipophilin expression in cutaneous malignant melanoma.

作者信息

Fujimoto Masakazu, Matsuzaki Ibu, Yamamoto Yuki, Yoshizawa Akihiko, Warigaya Kenji, Iwahashi Yoshifumi, Kojima Fumiyoshi, Furukawa Fukumi, Murata Shin-Ichi

机构信息

Department of Diagnostic Pathology, Wakayama Medical University, Wakayama, Japan.

Department of Dermatology, Wakayama Medical University, Wakayama, Japan.

出版信息

J Cutan Pathol. 2017 Mar;44(3):228-236. doi: 10.1111/cup.12868. Epub 2016 Dec 22.

DOI:10.1111/cup.12868

PMID:27886404

Abstract

BACKGROUND

The lipogenic pathway is upregulated in cancer cells, including melanomas. However, the pathological significance of cellular lipids in melanocytic lesions has yet to be determined. In this study, we evaluated intracytoplasmic lipid droplets in melanocytic nevi (MNs) and malignant melanomas via immunohistochemical analysis of adipophilin (ADP), which coats lipid droplets.

METHODS

One hundred primary cutaneous melanocytic lesions [33 MNs, 17 melanomas in situ (MIS), and 50 invasive melanomas (IMs)] were immunostained for ADP. The intensity score (IS) and proportion score (PS) of ADP staining in each case was recorded semiquantitatively on a scale of 0 to 3+.

RESULTS

High ADP expression (IS2/3+ and PS2/3+) was observed in 27 primary cutaneous melanocytic lesions that consisted of 23 IMs, three MISs, and one MN. Consequently, high ADP expression was associated with malignancy (38.8% vs. 3.0%; p < 0.0001). Among the IMs, high ADP expression was more prevalent in pT3/4 than pT1/2 (63.3% vs. 23.8%; p = 0.01) and Stage 3/4 than Stage 1/2 (76.9% vs. 36.8%; p = 0.02).

CONCLUSIONS

The majority of the melanocytic lesions with high ADP expression were malignant melanomas in our cohort. Therefore, ADP expression may serve as a sensitive diagnostic marker for malignant melanoma.

摘要

背景

包括黑色素瘤在内的癌细胞中脂肪生成途径上调。然而，黑素细胞性病变中细胞脂质的病理意义尚未确定。在本研究中，我们通过对包被脂滴的亲脂素（ADP）进行免疫组化分析，评估黑素细胞痣（MNs）和恶性黑色素瘤中的胞浆内脂滴。

方法

对100例原发性皮肤黑素细胞性病变[33例MNs、17例原位黑色素瘤（MIS）和50例浸润性黑色素瘤（IMs）]进行ADP免疫染色。对每例病例中ADP染色的强度评分（IS）和比例评分（PS）进行半定量记录，范围为0至3+。

结果

在27例原发性皮肤黑素细胞性病变中观察到高ADP表达（IS2/3+和PS2/3+），这些病变包括23例IMs、3例MIS和1例MN。因此，高ADP表达与恶性肿瘤相关（38.8%对3.0%；p<0.0001）。在IMs中，pT3/4期的高ADP表达比pT1/2期更常见（63.3%对23.8%；p = 0.01），3/4期比1/2期更常见（76.9%对36.8%；p = 0.02）。