Noorbakhshnia Maryam, Karimi-Zandi Leila
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Physiol Behav. 2017 Feb 1;169:69-73. doi: 10.1016/j.physbeh.2016.11.027. Epub 2016 Nov 24.
There is a growing body of evidence that neuroinflammation can impair memory. It has been indicated that Portulaca oleracea Linn. (POL), possess anti-inflammatory activity and might improve memory disruption caused by inflammation. In this study the effect of pre-treatment with the hydro-alcoholic extract of POL on memory retrieval investigated in lipopolysaccharide (LPS) treated rats. Male Wistar rats (200-220g) received either a control diet or a diet containing of POL (400mg/kg, p.o.) for 14days. Then, they received injections of either saline or LPS (1mg/kg, i.p.). In all the experimental groups, 4h following the last injection, passive avoidance learning (PAL) and memory test was performed. The retention test was done 24h after the training and then the animals were sacrificed. Hippocampal TNF-α levels measured using ELISA as one criteria of LPS-induced neuroinflammation. The results indicated that LPS significantly impaired PAL and memory and increased TNF-α levels in hippocampus tissue. Pre-treatment with POL improved memory in control rats and prevented memory and TNF-α deterioration in LPS treated rats. Taken together, the results of this study suggest that the hydro-alcoholic extract of POL may improve memory deficits in LPS treated rats, possibly via inhibition of TNF-α and anti-inflammatory activity.
越来越多的证据表明，神经炎症会损害记忆力。有研究表明，马齿苋具有抗炎活性，可能改善由炎症引起的记忆障碍。在本研究中，探讨了马齿苋水醇提取物预处理对脂多糖（LPS）处理的大鼠记忆恢复的影响。雄性Wistar大鼠（200 - 220g）接受对照饮食或含马齿苋的饮食（400mg/kg，口服），持续14天。然后，它们接受生理盐水或LPS（1mg/kg，腹腔注射）注射。在所有实验组中，最后一次注射后4小时，进行被动回避学习（PAL）和记忆测试。训练后24小时进行记忆保持测试，然后处死动物。使用ELISA测量海马组织中TNF-α水平，作为LPS诱导神经炎症的一个指标。结果表明，LPS显著损害PAL和记忆力，并增加海马组织中TNF-α水平。马齿苋预处理改善了对照大鼠的记忆力，并防止LPS处理大鼠的记忆力和TNF-α恶化。综上所述，本研究结果表明，马齿苋水醇提取物可能通过抑制TNF-α和抗炎活性改善LPS处理大鼠的记忆缺陷。
