Xu Shan, Chan Rachel W S, Ng Ernest H Y, Yeung William S B
Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong, SAR, China.
Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong, SAR, China; Centre of Reproduction, Development of Growth, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China.
Exp Cell Res. 2017 Jan 1;350(1):184-189. doi: 10.1016/j.yexcr.2016.11.020. Epub 2016 Nov 24.
The human endometrium is a highly dynamic tissue with the ability to cyclically regenerate during the reproductive life. Endometrial mesenchymal stem-like cells (eMSCs) located throughout the endometrium have shown to functionally contribute to endometrial regeneration. In this study we examine whether the menstrual cycle stage and the location in the endometrial bilayer (superficial and deep portions of the endometrium) has an effect on stem cell activities of eMSCs (CD140bCD146 cells). Here we show the percentage and clonogenic ability of eMSCs were constant in the various stages of the menstrual cycle (menstrual, proliferative and secretory). However, eMSCs from the menstrual endometrium underwent significantly more rounds of self-renewal and enabled a greater total cell output than those from the secretory phase. Significantly more eMSCs were detected in the deeper portion of the endometrium compared to the superficial layer but their clonogenic and self-renewal activities remained similar. Our findings suggest that eMSCs are activated in the menstrual phase for the cyclical regeneration of the endometrium.
人类子宫内膜是一种高度动态的组织，在生殖期具有周期性再生的能力。遍布子宫内膜的子宫内膜间充质干细胞样细胞（eMSCs）已被证明在功能上有助于子宫内膜再生。在本研究中，我们研究月经周期阶段以及子宫内膜双层（子宫内膜的浅层和深层）中的位置是否对eMSCs（CD140bCD146细胞）的干细胞活性有影响。在此我们表明，eMSCs的百分比和克隆形成能力在月经周期的各个阶段（月经期、增殖期和分泌期）是恒定的。然而，来自月经内膜的eMSCs比来自分泌期的eMSCs经历了更多轮的自我更新，并产生了更大的总细胞产量。与浅层相比，在子宫内膜深层检测到的eMSCs明显更多，但其克隆形成和自我更新活性保持相似。我们的研究结果表明，eMSCs在月经期被激活以实现子宫内膜的周期性再生。
