Zhao Hongxia, Chen Xiuying, Shen Chen, Qu Baocheng
a Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology , Dalian University of Technology , Dalian , China.
b Division of Food Detection , Dalian Institute of Food Inspection , Dalian , China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 Feb;34(2):255-264. doi: 10.1080/19440049.2016.1266096. Epub 2016 Dec 14.
A simple and efficient method for determining multiple mycotoxins was developed using a QuEChERS (quick, easy, cheap, effective, rugged and safe)-based extraction procedure in vegetable oils. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used for the quantification and confirmation of 16 chemically diversified mycotoxins. Different extraction procedures were studied and optimised by spiking 16 analytes into blank matrix, and the extraction with 85% MeCN solution and C18 as cleaning sorbent allowed an efficient recovery of 72.8-105.8% with RSDs less than 7%. The limit of detection (LOD) ranged from 0.04 to 2.9 ng g. The developed method was finally applied to screen mycotoxins in 62 vegetable oil samples. Zearalenone (ZEN), aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and α-zearalenol (α-ZOL) were detected, with maximum concentrations of 0.59 (AFG1)-42.5 (ZEN) ng g. The method developed has the advantages of high sensitivity, accuracy and selectivity, and it can be applied to the target screening of mycotoxins in real samples.
建立了一种简单高效的测定多种霉菌毒素的方法，该方法采用基于QuEChERS（快速、简便、廉价、有效、耐用且安全）的植物油提取程序。采用高效液相色谱-串联质谱法（HPLC-MS/MS）对16种化学性质不同的霉菌毒素进行定量和确证。通过向空白基质中添加16种分析物，研究并优化了不同的提取程序，以85%乙腈溶液作为提取剂、C18作为净化吸附剂时，回收率为72.8% - 105.8%，相对标准偏差小于7%。检测限范围为0.04至2.9 ng g。所建立的方法最终应用于62个植物油样品中霉菌毒素的筛查。检测到了玉米赤霉烯酮（ZEN）、黄曲霉毒素B1（AFB1）、黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）和α-玉米赤霉醇（α-ZOL），最大浓度为0.59（AFG1）- 42.5（ZEN）ng g。所建立的方法具有灵敏度高、准确性高和选择性好的优点，可应用于实际样品中霉菌毒素的靶向筛查。
